Basal construction studies for the diversity of algae-protist complexes

了解水生生态系统中真核微生物多样性的建立、维持和崩溃的动态，以及这些生物之间的相互作用和生物与环境有机物之间的相互关系，是水生微生物生物学的重要问题。为了评估决定这些动态的因素，我们进行了代谢组学、宏基因组学和叶绿素衍生物的分析，以及微观观察。我们发现一些有机物的数量随生物群系的时空变化而增加或减少。我们还发现了一种代谢物，可以作为检测原生生物捕食活性的新生物标志物。此外，利用已建立的培养物对需要重新分类评估的藻类和原生生物进行了检查。

Understanding the dynamics of establishment, maintenance and collapse of the diversity of eukaryotic microorganisms in aquatic ecosystems, and the interaction among those organisms and interrelation between the organisms and environmental organic matter, are important issues of aquatic microbial biology. To evaluate factors that determine these dynamics, we conducted analyses of metabolomics, metagenomics and chlorophyll derivatives, along with microscopic observations. We identified some organic matter whose quantities increased or decreased coupled with the spatio-temporal changes of the biome. We also found a metabolite that can be a new biomarker to detect the predatory activity of protists. Furthermore, algae and protists requiring taxonomic re-evaluation were examined using established cultures.