Molecular analysis of localization of biliary transporters in chronic hepatitis

在这项研究中，我们通过人体肝脏切片检查了慢性肝炎患者胆道转运蛋白的定位和生理性肝衰竭。利用Ethan DIGE进行二维荧光差分分析，可以确定胆道转运蛋白的新结合伙伴（AnexinA2等）。我们可以表征AnexinA2调控Mrp2的小管膜定位。此外，胆道转运蛋白之一Bsep的抑制剂（CyclopsorinA和Glibenclamide）在人肝细胞夹层培养的浓缩胆汁酸混合物中显示出毒性。

In this study, we examined localization of biliary transporters and physiological liver failure in chronic hepatitis by using human liver slices. We could determine the new binding partners (AnexinA2 etc) of biliary transporters by using two-dimensional fluorescence differential analysis with Ethan DIGE. And we could characterize AnexinA2 regulate the canalicular membrane localization of Mrp2. Moreover, inhibitors (CyclopsorinA and Glibenclamide) of Bsep, one of biliary transporter, shows toxicity in the presence of the concentrated bile acids mixtureby using human sandwich cultured hepatocytes.