The effect of sex hormone on canilaae metabolism

性激素对软骨代谢的影响尚不清楚，雌激素对软骨分化的影响也未见报道。雌激素是维持成骨细胞和成骨细胞活性之间的平衡在成人骨重建过程中必不可少的。为了研究雌激素增强软骨形成分化的机制，使用ATDC 5细胞（小鼠前软骨形成细胞系）和原代培养的小鼠肋骨软骨形成细胞进行体外实验。软骨结节的阿辛蓝染色和II型胶原编码基因表达的实时逆转录聚合酶链反应。X型胶原蛋白和Augrecan在用雌激素处理的细胞中进行。细胞中聚集蛋白聚糖和II型胶原蛋白的表达水平（软骨形成分化的标志物）通过低剂量雌激素（10 μ M）持续升高<-12>，并通过高剂量（10 μ <-6>M）抑制。ATDC 5和原代培养的软骨细胞经一定浓度的雌激素处理后，Col II和聚集蛋白聚糖表达增加。

The effect of sex hormone on cartilage metabolism is unclear, and little has been reported about the effects of estrogen on the cartilage differentiation. Estrogen is essential for maintaining the balance between osteoblastic and osteoclastic activity during bone remodeling in adults. To investigate the mechanism by which estrogen enhances chondrogenic differentiation, in vitro experiments were carried out using ATDC5 cells(a mouse pre-chondrogenic cell line) and primary cultured chondrogenic cells of mouse rib. Alcian blue staining for cartilage nodules and real-time reverse-transcription polymerase chain reaction for the expression of genes encoding type II collagen. type X collagen and augrecan, were performed in cells treated with estrogen. The expression level of aggrecan and type II collagen in cells, a marker of chondrogenic differentiation, was consistently elevated by low dose of estrogen(10^<-12> M) and was suppressed by high dose(10^<-6> M). ATDC5 and primary cultured chondrogenic cells treated with certain concentration of estrogen have higher expression of Col II and aggrecan.