Gentic control of migration

SF/HGF及其受体c-met对于肌源性前体迁移的第一步至关重要，即上皮真皮组织细胞的分层。携带Lbx1基因突变或削弱c-met信号传导能力（c-methypo和Gab1）突变的小鼠已经可用。它们允许前体分层，但干扰随后的迁移。初步实验表明，在某些方面，Gab1和Lbx1突变具有相似的表型。双突变体将允许表征是否两个基因接管部分冗余的功能。神经调节蛋白及其受体erbb B2和erbb B3是神经嵴细胞正确迁移所必需的，但不是神经嵴细胞从神经管剥离所必需的。为了分析细胞是否需要信号进行定向迁移或维持其迁移潜力，我们将在神经嵴细胞中以自分泌方式表达神经调节蛋白。此外，外植体培养将用于研究神经调节蛋白对迁移过程的影响。

SF/HGF and its receptor c-met are essential for the first step in the migration of myogenic precursors, the delamination of the cells from the epithelial dermomyotome. Mice that carry mutations in the Lbx1 gene, or mutations that attenuate the c-met signaling capacity (c-methypo and Gab1) have become available. They allow delamination of the precursors, but interfere with subsequent migration. Preliminary experiments indicate that, in some respects, the Gab1 and the Lbx1 mutation have similar phenotypes. Double mutants will allow to characterize whether the two genes take over partially redundant functions. Neuregulin and its receptors, erb B2 and erb B3, are required for correct migration of neural crest cells, but not for their delamination from the neural tube. To analyze whether the cells require the signal for directed migration or for the maintenance of their migratory potential, we will express neuregulin in an autocrine manner in neural crest cells. Moreover, explant cultures will be used to study the effect of neuregulin on the migration process.