Regulation of heat shock response, maltose transport, pyrimidine synthesis and ribosomal RNA synthesis in Pyrococcus furiosus

本实验室初步鉴定了焦球菌的两种转录调节因子。亮氨酸反应调节蛋白（LRP）和一个24 kDa的热休克调节蛋白。这些成分与基础转录机制的相互作用及其作用机制将在分子细节上进行研究。dna -蛋白质相互作用将通过各种足迹技术进行研究，蛋白质-蛋白质相互作用将在体外和体内进行研究。将确定热冲击调节器的晶体结构，无论是单独的还是与目标DNA的复合结构。将分析NTP浓度对各种启动子转录的影响，这些启动子引导嘧啶合成蛋白的表达。*

Two transcriptional regulators of Pyrococcus have been characterized preliminarily in this laboratory. The Leucine responsive regulatory protein (LRP) and a 24 kDa regulator of a heat shock regulon. The interaction of these components with the basal transcriptional machinery and their mechanism of action will be studied in molecular detail. DNA-protein interactions will be investigated by various footprinting techniques, protein-protein interactions will be studied in vitro and in vivo. The crystal structure of the heat shock regulator both alone and in complex with its target DNA will be determined. The effect of NTP concentration on transcription of various promoters directing expression of proteins involved in pyrimidine synthesis will be analysed. *