Practical strategies for engineering the CO2-fixing enzyme, Rubisco, whose subunits are encoded in different subcellular compartments.

我最近用一种效率较低的细菌版本取代了植物固定二氧化碳的酶Rubisco，用一种由单一基因编码的单一亚基类型，证明了取代Rubisco的可行性。这鼓励人们继续尝试用更高效的版本来取代植物Rubisco，这样植物就可以在更少的水、肥料或光照下生长。最有效的RuBiscos更复杂，在植物中，有两种不同类型的亚基，编码在不同的亚细胞隔间(核和叶绿体)。这项建议解决了对两个基因组进行互补工程以将外来RuBiscos替换为高等植物叶绿体所面临的挑战。

My recent replacement of the plant CO2-fixing enzyme, Rubisco, with a less efficient bacterial version, with a single type of subunit encoded by a single gene, demonstrated the feasibility of replacing Rubisco. This encourages ongoing attempts to replace plant Rubisco with more efficient versions that would allow the plants to grow with less water, fertiliser or light. The most efficient Rubiscos are more complex, with two different types of subunits which, in plants, are encoded in different subcellular compartments (nucleus and plastid). This proposal addresses the challenges associated with complementary engineering both genomes to substitute foreign Rubiscos into higher-plant chloroplasts.