Cell Sorter

我们在此申请采购一种用户友好的细胞分选机，作为一种台式设备，可以轻松分选各种细胞大小，以满足不同的应用。我们是一群年轻而创新的研究小组，拥有蒂宾根大学的基本资助，并成功获得欧洲研究理事会或德国研究基金会的资助。事实上，我们所有人都在国外呆了很长一段时间（费赫特和莱博尔德在纪念斯隆-凯特琳癌症中心，舒尔希在斯坦福大学，弗拉茨在美国国立卫生研究院）。除了免疫学之外，我们还接受了图宾根的职位，通过基础和转化研究果断地推进我们的领域。在这样做的过程中，作为临床科学家，我们多次成功地承担了验证我们在人体样本基础研究中的发现的任务，从而使研究更接近临床或患者的需求。从我们的日常临床工作中，我们知道目前诊断和治疗的局限性。由于我们都在很大程度上使用难以获得的人体样本，因此我们必须日夜和周末直接使用可以由我们的实验室成员独立操作的细胞分选设备。短距离还可确保样品保持新鲜，以便在进行过程中进行扩展，并可用于进一步的实验。因此，仪器的一个重要标准是尽可能完全自动化设置、采集、分选和分析，以便分选易于执行，从而独立于操作员。系统软件应直观，并支持分类到试管和96孔板和384孔板中。原始分选数据应在FCS 3.0和FCS 3.1文件中可用，以便可以将其导出到第三方分析工具。所述两种器械均符合这些质量标准。获得这样的设备将大大加快新数据的收集，并改善团队之间的协作。这些设备将帮助我们与世界领先的实验室进行实验，并巩固蒂宾根的研究基地。这对于未来招募更多有才华的年轻研究人员也至关重要。

We hereby apply for the procurement of a user-friendly cell sorter which, as a benchtop device, enables the easy sorting of a wide range of cell sizes for diverse applications. We are a cluster of young and innovative research groups that have basic funding from the University of Tübingen and have also been successful in obtaining funding from the European Research Council or the German Research Foundation. Virtually all of us have been abroad for extended periods of time (Feucht & Leibold at Memorial Sloan Kettering Cancer Center, Schürch at Stanford University, and Flatz at the National Institutes of Health). In addition to immunology, we are united by the fact that we accepted a position in Tübingen with the vision of decisively advancing our fields through basic and translational research. In doing so, as clinician-scientists, we repeatedly successfully take on the task of verifying our discoveries in basic research on human samples, thus bringing research closer to the needs of the clinic or the patient. From our daily clinical work, we know the current limitations of diagnostics and therapeutics. Since we all work to a large extent with human samples that are difficult to obtain, it is crucial that we have direct access day and night and on weekends to a cell sorting device that can be operated independently by our laboratory members. A short distance also ensures that the samples remain fresh so that they can be expanded as they progress and are available for further experiments. Therefore, an important criterion for the instrument is to automate setup, acquisition, sorting, and analysis as completely as possible so that sorting is easy to perform and thus independent of an operator. The system software should be intuitive and support sorting into tubes and 96- and 384-well plates. Raw sorting data should be available in FCS 3.0 and FCS 3.1 files so that they can be exported to third-party analysis tools. These specifications are met by the two devices described. Obtaining such a device will greatly speed up the collection of new data and improve collaboration among teams. Such equipment will help us to perform experiments on par with world-leading laboratories and consolidate the research location in Tübingen. This will also be crucial for recruiting more talented young researchers in the future.