In situ analysis for the molecular recognition on solid materials

1998年，我们对固体表面的选择剂分子进行了一些基础性的研究。利用硅胶表面的选择剂分子，成功地进行了一些色谱研究。其次，我们设计了一些新的固液界面分析方法。这些方法探测固液界面的第一层或固体表面附近。方法如下。1)一种新的全内反射结构的深度分辨热透镜光谱学。2)表面等离子体共振。这些方法探测在倏逝场范围内固体附近的动态分子事件。3)利用Nd/YAG激光系统设计了表面固有二次谐波发生装置。1998年，我们改进了这些方法，并进行了色谱研究，以了解分子间相互作用的强度。利用热透镜的全内反射几何分析方法，研究了液相中有机染料在玻璃表面的吸收现象。为了高灵敏度和高选择性地探测固/液界面第一层，我们采用YAG激光器和染料激光器相结合的方法，构建了一台共振s-SHG装置。偶氮苯在紫外光照射下会从顺式异构化，而在可见光或热作用下会还原成反式。利用反式偶氮苯的共振条件，建立了偶氮苯在界面上的光交替动力学。深度分辨固体结构是界面分子识别的另一个重要因素，采用新型脉冲激光诱导光声光谱法成功探测了深度分辨固体结构。用高效液相色谱法和毛细管电泳法研究了固/液界面的分子间相互作用。这些研究系列正在发展为与西奈山医学院J.B.A.Ross教授的国际合作。

In 1998, we have made some basic research concerning to the selector molecules on solid surface. By using the selector molecules on silica-gel surface, some chromatographic studies were carried out successfully. Second, we devised some novel analytical methods for solid liquid interface. Those methods probe the first layer of solid liquid interface or in the vicinity of the solid surface. The methods are as follows. 1) A novel depth resolving thermal lens spectroscopy of total internal reflection geometry. 2) Surface plasmon resonance. These methods probe dynamic molecular events in the vicinity of solid in the range of evanescent field. 3) The instrument for surface inherent second harmonic generation was devised by Nd/YAG laser system. In 1998, we have improved these methods and also carried out chromatographic studies for the understanding of the intensity of molecular-molecular interaction. Using thermal lens analysis of total internal reflection geometry, we made the absorptionphenomena of organic dye in the liquid phase to the glass surface. To probe the first layer of the solid / liquid interface with high sensitivity and selectivity, we constructed a resonant s-SHG instrument by the combination of YAG laser and dye laser. Azobenzene connected on glass surface was known to isomenze from cis form by UV light irradiation and to return to its original trans form by visible light or heat. By using resonant condition for trans form of a.zobenzene, we made the photo alternate dynamics of azobenzene at the interface. Depth resolving solid structure, which is another important factor for molecular recognition at the intefface, was successfully probed by novel pulse laser induced photoacoustic spectrometry. Molecular interaction at the solid/liquid interface was also studied by HPLC and capillary electrophoresis. The series of these studies are developing to international collaborative work with Professor J.B.A.Ross, Mt.Sinai School of Medicine.