Influence of repeated administration of proline analogue to weanling rats

结合Emery（1970）和我们之前的实验（Kida et al 1980, 1981）的观察结果，胶原蛋白和弹性蛋白在出生后肺泡形成中起着关键作用。在本实验方案中，断奶大鼠分别接受脯氨酸类似物（包括顺式4-羟基脯氨酸和氨基脲）治疗，并将其形态学和压力曲线测量结果与对照动物进行比较。顺式4-羟基脯氨酸处理后，肺功能和肺形态均无明显变化，只是体重较对照组大。氨基脲治疗大鼠胸壁明显畸形，正常肺泡形成受到抑制。由此，我们认为胸壁运动在出生后肺泡生长中起着额外的作用，因此，我们制作了胸壁受限大鼠模型，并对其切除肺的肺功能和形态学进行了研究。肺内肺泡数量明显减少，但肺泡大小变化幅度不明显。

Together with observations by Emery (1970) and our previous experiments (Kida et al 1980, 1981), collagen and elastin play a key role in postnatal alveolar formation in this experimental protocol, weanling rats were treated with proline analogues which including cis-4-hydroxyproline and semicarbazide, respectively, and their morphology and pressure-curve measurements were compared with these of control animals. The lungs of cis-4-hydroxyproline treated animals did not reveal any remarkable changes in the lung function and morphology, respectively, except for the larger body weight than the control animals. Semicarbazide treated rats showed marked deformity of chest wall and showed surpression of normal alveolar formation. From these observation, chest wall movements was considered an additional role in the postnatal alveolar growth, Therefore, a rat model of chest wall restriction was made and their excised lungs was studied in term of lung function and morphology. These rats lung showed remarkable decreased of number of alveoli in the lung but each alveolar sizxe did not change in significant range.