Immunocytochemical studies on DMBA induced lingual carcinoma in hamster

本实验用免疫组织化学方法研究了中等大小的胞囊微丝在肿瘤形成过程中的分布发展及其对辐射的影响。结果1.在正常和反应期，细胞角蛋白(CK)在所有鳞状细胞层中均可检测到。浅层有角蛋白-鳞状上皮-单克隆性(SE)和角蛋白-非鳞状上皮-单克隆性(NSE)，浅层和中间层有Involucrin(Inv)阳性反应。而Vimentin(Vim)在各层均为阴性。在发生的鳞状细胞癌中，角蛋白表现为抗体活性的不规则和马赛克的混合图像。随着角化程度的加深，反映鳞状细胞分化的CK染色逐渐增强，一组细胞在…的周边可见CK的定位染色胞质和胞核较多。INV染色在角化部外层附近的分化区呈阳性，在反应区呈不确定趋势。未分化区VIM染色均为阳性。放疗组。辐照组和非辐照组之间的染色能力无明显差异。实验细胞学标本SE呈弥漫性阳性反应，分布于整个肿瘤细胞质。但未检测到染色的定位。在临床恶性病例的细胞学标本中，所有病例均呈SE强染色，未分化病例呈Vim阳性。结果表明，角蛋白有助于判断鳞状细胞的起源，Vim可作为鳞状细胞分化的标志。但没有出现阳性病例，可能反映了实验中的少数病例。因此，我们的研究还有待于进一步探讨这一遗留问题。较少

The experimental study was undertaken to examine the development of the distribution of intermediate-sized cytoskeltal filaments in the process of cancer formation and the radiation effect immunohistochemically. Supplementary to the experiments, clinical application were performed.Result 1. In normal and reacative stages, Cytokeratin (CK) was detectable in all of the squamous cell layers. Keratin-Squamous Epithelium-monoclonal (SE) and Keratin-Nonsquamous Epithelium-monoclonal (NSE) were observed in the superficial layer and Involucrin (Inv) positive reaction was seen in the superficial and the intermediate layers. But Vimentin (Vim) was negative in all layers.2. In the cases of the developed squamous cell carcinoma, keratin showed the mixed images of irragular and mosaic pattern of the antibody activity. CK staining became strong with the advancement of keratinization reflecting the squamous differentiation and a group fo cells showed the localized staining of CK at the periphery fo t … More he cytoplasm and nucleus. Inv stainig was positive in the differentiated area close to the outer layer of the keratinized portion showing a difinite tendency in the reacting area. Vim staning was positive throughout the undifferentiated area.3. In radiation group. There were no apparent differences in stainability between the rediated and the non-radiated groups.4. The experimental cytologic specimens SE was shown as diffuse positive reactive image in the entire tumor cytoplasm. But the localization of the staining could not be detected.5. In the cytologic specimens from clinicl malignant cases, tunor cells were strongly stained by SE in all cases and Vim was postitve in undifferenciated cases. The results showed that keratin was useful to decide the squamous origin and Vim as a marker for squamous differentiation. But there were no positive cases of Inv whihc might reflect a small number of cases in the experiment. Therefore, our study should be continued further to discuss this remained probem. Less