Effect of physical exercise on the permeability of the cellular membrane to enzymes and its mechanism

目前的研究的目的是为了调查物理练习对细胞膜的渗透性的影响。接下来的三个实验是在做的： 1。跑步训练：10周物理训练的影响（跑步超过5公里， 6次/周）在七名高级男性学生中的活动和集中<gamma>-谷氨酰胺转肽酶（<gamma>GTP）。在训练了保留等离子体<gamma>GTP集中度后，当<gamma>GTP活动没有实质性地进行。以下建议是，在培训<gamma>GTP的预校准形式没有出现在等离子体中，而“激活蛋白”出现在等离子体中，很可能会导致像肝脏这样的组织泄漏。2.进行的练习：15只雄性Wistar老鼠在游泳前被锻炼，在35 ° C的水中重量等于3%。微<gamma>GTP是在肝脏细胞片段中研究的。在体内和体外<gamma>渗透率（%）= （体外渗透率x 100 ）/（体外渗透率+微观渗透率）和体外渗透率（%）= （体外渗透率）/（体外渗透率）/（体外渗透率）x100在体内渗透率（%） = （体外渗透率）/（体外渗透率） x 100在游泳后对体外渗透率的显著<gamma>增强，但在游泳后的体外渗透率没有显著改变。这一指标表明，急性锻炼增加了大鼠肝脏微膜的普遍性。3.游泳训练：十只小白鼠在35 ° C的水中游泳10周，每天游泳3小时，5次/周。在体内和体外的渗透能力-通过<gamma>GTP和内容估计-活体微细胞的成员出现在减少，并建议增加对三微细胞膜的稳定性。

The purpose of the present study was to investigate the influence of physical exercise on the permeability of the cellular membrane to enzymes. The following three experiments were conducted: 1. Running training: the effects of 10-week physical training (running over 5 km, 6 times/week) on both the activity and concentration of <gamma>-glutamyl transpeptidase (<gamma>GTP) in plasma were investigated on seven sedentary male students. After training the resting plasma <gamma>GTP concentration decreased to half, whereas the <gamma>GTP activity did not vary substantially. This suggests that after training the precursor form of <gamma>GTP did not appear in the plasma and that 'activator protein' appeared in the plasma, possibly due to a leakage from the tissues such as liver. 2. Prolonged exercise: Fifteen male Wistar rats were exercised by swimming until exhausted with a weight equal to 3 % of their body weight in water at 35゜C. Microsomal <gamma>GTP was studied in the liver cell fractions. In vivo and in vitro permeabilities for both <gamma>GTP activity and content were calculated by the following formulas:In vivo permeability (%)= (extramicrosomal fraction x 100 )/(tramicrosomal fraction + microsomal fraction ) andin vitro permeability (%) = ( hyposmotic condition )/(osmotic condition ) x 100The in vivo permeability of both <gamma>GTP activity and content was greatly enhanced after swimming, whereas the in vitro permeability did not change significantly. This indicates that acute exercise increases the permeability of the microsomal membrane of rat liver. 3. Swimming training: Ten male Wister rats were exercised by swimming to 3 h/day, five times/week for 10 weeks in water at 35゜C. After training both the in vivo and in vitro permeabilities-estimated by <gamma>GTP and content-of the membrane of the liver microsomes appeared to reduce, suggesting an increased stability of thr microsomal membrane.