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The response of adipose tissues to oxidative stress by exercise : with special reference to the role of EC-SOD in splanchnic adioise tissue

脂肪组织对运动引起的氧化应激的反应：特别参考 EC-SOD 在内脏脂肪组织中的作用

基本信息

批准号：
17300211
负责人：
OHNO Hideki
金额：
$ 9.79万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

The main findings obtained during 2005-2006 are as follows:1. The effect of acute exercise on β-adrenergic receptor (β-AR) trafficking was investigated in rat adipocytes. As the result, the mechanism underlying the increased density of β_2-AR up to at least 3 h after exercise might involve alterations in a multistep event involving the coordinate interaction among proteins mediating β_2-AR trafficking, in which both the receptor-agonist interactions and ubiquitin-proteasome pathway have a key role. However, the decreased protein expression of β_2-AR at 24 h might be due to some change occurring at the translational levels.2. The effect of exercise training on oxidative stress was investigated in rat adipocytes. In consequence, the level of thiobarbituric acid-reactive substances (TBARS) was significantly decreased in splanchnic adipose tissue, but not in peripheral adipose tissue. Meanwhile, extracellular superoxide dismutase (EC-SOD) level appeared to increase only in splanchnic adipo … More se tissue. Considering reduced level of monocyte chemoattractant protein-1 (MCP-1) in splanchnic adipose tissue after training, the findings obtained suggest that physical training reduces oxidative stress in splanchnic adipose tissue, resulting in improvement in lifestyle-related disease.3. The antioxidative effect of new lychee-derived polyphenol Oligonol converted into a low-molecular form (Amino Up Chemical Co., Ltd., Sapporo) was investigated in mouse adipocytes. Although the levels of splanchnic adipose tissue mass and TBARS in mice given a high fat diet (HFD) were significantly higher than those in control mice, Oligonol definitely inhibited such HFD-induced increases. Expression of TNF-α and MCP-1 genes in splanchnic adipose tissue of HFD mice was also significantly higher than that in control and HFD + Oligonol mice. Moreover, the expression of EC-SOD gene was enhanced only in splanchnic adipose tissue of HFD + Oligonol mice. These results suggest that Oligonol has favorable effects on oxidative stress. Less

2005-2006年期间取得的主要成果如下：1.本文研究了急性运动对大鼠脂肪细胞β-肾上腺素能受体（β-AR）运输的影响。结果表明，运动后至少3 h内β 2-AR密度增加的机制可能与调节β 2-AR运输的蛋白质之间协同作用的多步骤事件有关，其中受体-激动剂相互作用和泛素-蛋白酶体途径起关键作用。而β_2-AR在24 h的蛋白表达下降可能是由于翻译水平发生了变化.研究了运动训练对大鼠脂肪细胞氧化应激的影响。因此，硫代巴比妥酸反应物质（TBARS）的水平显着降低内脏脂肪组织，但不是在外周脂肪组织。同时，细胞外超氧化物歧化酶（EC-SOD）水平仅在内脏脂肪组升高， ...更多信息 se组织。考虑到训练后内脏脂肪组织中单核细胞趋化蛋白-1（MCP-1）水平的降低，所获得的研究结果表明，体育训练降低了内脏脂肪组织中的氧化应激，从而改善了生活方式相关疾病.转化成低分子形式的新的荔枝衍生的多酚Oligonol（Amino Up Chemical Co.，有限公司，札幌）在小鼠脂肪细胞中进行了研究。尽管给予高脂饮食（HFD）的小鼠的内脏脂肪组织质量和TBARS水平显著高于对照小鼠，但Oligonol明确抑制了这种HFD诱导的增加。HFD小鼠内脏脂肪组织中TNF-α和MCP-1基因的表达也显著高于对照组和HFD + Oligonol小鼠。HFD + Oligonol小鼠内脏脂肪组织EC-SOD基因表达增强。这些结果表明，Oligonol对氧化应激具有有利的作用。少