Can physical exercise induce differentiation from myoblasts to brown adipocytes ?

体育锻炼能否诱导成肌细胞分化为棕色脂肪细胞？

• 批准号: 22650154
• 负责人: OHNO Hideki
• 金额: $ 2.07万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650154/
• 关键词: 褐色脂肪細胞; 水泳運動; UCP-1; PRDM16; 骨格筋; Mfsd2a; BMP7; DNAアレイ; 寒冷曝露; βアドレナリン受容体; C/EBP-β; PGC-1α; 運動; 骨格筋筋芽細胞; 脱共役タンパク質; Cidea; カテコールアミン

Recently, there has been growing evidence that PRDM16 acts as a bidirectional switch to drive the differentiation of Myf-5-expressing mesenchymal stem cells into the brown adipocytes (BA). The aim of the current study was to test whether physical exercise modifies this differential event. Male C57BL/6J mice were randomly divided into 3 groups: the sedentary control group, the acute swimming group and the cold exposed group, a positive control of BA function. Several partsof skeletal muscles were used for analysis of both mRNA and protein which might be concerned with this event. Expression of mRNA for UCP-1, a specific marker of BA, was detected only in interscapular muscles in each group. Swimming group significantly increased PRDM16 mRNA and protein levels compared with control group. Moreover, levels of PRDM16 and C/EBP-β, which is a pivotal key regulator for switching of PRDM16, were significantly higher in swimming group than in control group. These results demonstrate that swimming exercise has powerful effects on differentiation event of Myf-5-expressed myoblasts into BA, thereby suggesting that swimming exercise is clinically useful as new anti-obesitytreatment.

最近，越来越多的证据表明PRDM 16作为双向开关驱动表达Myf-5的间充质干细胞分化为棕色脂肪细胞（BA）。本研究的目的是测试体育锻炼是否会改变这种差异事件。雄性C57 BL/6 J小鼠随机分为3组：安静对照组、急性游泳组和冷暴露组，以BA功能为阳性对照。对部分骨骼肌进行了可能与此事件有关的mRNA和蛋白质的分析。UCP-1的mRNA表达，BA的特异性标志物，仅在各组肩胛间肌中检测到。与对照组相比，游泳组PRDM 16 mRNA和蛋白水平显著升高。此外，游泳组PRDM 16和C/EBP-β的水平显著高于对照组，C/EBP-β是PRDM 16转换的关键调节因子。这些结果表明，游泳运动对表达Myf-5的成肌细胞向BA的分化事件具有强大的影响，从而表明游泳运动在临床上可用作新的抗肥胖治疗。

项目成果

Proceedings 19th International Congress on Nutrition and Integrative Medicine

第十九届国际营养与综合医学大会论文集

• 发表时间: 2011
• 作者: 櫻井拓也;ほか
• 通讯作者: ほか

新規低分子かポリフェノール(Oligonol)による脂肪分解反応の調節メカニズム

新型低分子量多酚（Oligonol）调节脂肪分解反应的机制

• 发表时间: 2010
• 作者: 小笠原準悦;他
• 通讯作者: 他

老化促進モデルマウスの認知機能障害に対する運動トレーニング効果

运动训练对加速衰老模型小鼠认知功能障碍的影响

• 发表时间: 2010
• 作者: 櫻井拓也;他
• 通讯作者: 他

Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue

运动训练对大鼠附睾白色脂肪组织基质血管成分细胞脂肪生成的影响

• DOI: 10.1111/j.1748-1708.2010.02159.x
• 发表时间: 2010
• 期刊: Acta Physiol(Oxf)
• 作者: Sakurai;T.;Endo;S.;Hatano;D.;Ogasawara;J.;Kizaki;T.;Oh-ishi;S.;Izawa;T.;Ishida;H. & Ohno;H.
• 通讯作者: H.

Oligonol, an oligomerized lychee fruit-derived polyphenol, activates the Ras/Raf-1/MEK1/2 cascade independent of the IL-6 signaling pathway in rat primary adipocytes.

• DOI: 10.1016/j.bbrc.2010.10.082
• 发表时间: 2010-11
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: J. Ogasawara;K. Kitadate;H. Nishioka;H. Fujii;T. Sakurai;T. Kizaki;T. Izawa;H. Ishida;M. Tanno;H. Ohno