Can physical exercise induce differentiation from myoblasts to brown adipocytes ?

最近，越来越多的证据表明PRDM 16作为双向开关驱动表达Myf-5的间充质干细胞分化为棕色脂肪细胞（BA）。本研究的目的是测试体育锻炼是否会改变这种差异事件。雄性C57 BL/6 J小鼠随机分为3组：安静对照组、急性游泳组和冷暴露组，以BA功能为阳性对照。对部分骨骼肌进行了可能与此事件有关的mRNA和蛋白质的分析。UCP-1的mRNA表达，BA的特异性标志物，仅在各组肩胛间肌中检测到。与对照组相比，游泳组PRDM 16 mRNA和蛋白水平显著升高。此外，游泳组PRDM 16和C/EBP-β的水平显著高于对照组，C/EBP-β是PRDM 16转换的关键调节因子。这些结果表明，游泳运动对表达Myf-5的成肌细胞向BA的分化事件具有强大的影响，从而表明游泳运动在临床上可用作新的抗肥胖治疗。

Recently, there has been growing evidence that PRDM16 acts as a bidirectional switch to drive the differentiation of Myf-5-expressing mesenchymal stem cells into the brown adipocytes (BA). The aim of the current study was to test whether physical exercise modifies this differential event. Male C57BL/6J mice were randomly divided into 3 groups: the sedentary control group, the acute swimming group and the cold exposed group, a positive control of BA function. Several partsof skeletal muscles were used for analysis of both mRNA and protein which might be concerned with this event. Expression of mRNA for UCP-1, a specific marker of BA, was detected only in interscapular muscles in each group. Swimming group significantly increased PRDM16 mRNA and protein levels compared with control group. Moreover, levels of PRDM16 and C/EBP-β, which is a pivotal key regulator for switching of PRDM16, were significantly higher in swimming group than in control group. These results demonstrate that swimming exercise has powerful effects on differentiation event of Myf-5-expressed myoblasts into BA, thereby suggesting that swimming exercise is clinically useful as new anti-obesitytreatment.