Role of clock genes in macrophages in the crosstalk between exercise and immunity

小鼠巨噬细胞系RAW264细胞过度表达Rev-erb抑制LPS内毒素刺激后Ccl2的诱导，导致细胞粘附和迁移活性受损。此外，Rev-erb通过Ccl2启动子区域的Rev-erb结合基序直接抑制Ccl2的表达。这些发现表明Rev-erb通过直接调节Ccl2的表达来调节巨噬细胞的炎症功能。

Overexpression of Rev-erb in murine macrophage cell line RAW264 cells suppressed the induction of Ccl2 following an LPS endotoxin challenge, resulting in the impaired activity of cell adhesion and migration. In addition, Rev-erb represses Ccl2 expression directly through a Rev-erb binding motif in the Ccl2 promoter region. These findings demonstrate that Rev-erb modulates the inflammatory function of macrophages through the direct regulation of Ccl2 expression.