Role of clock genes in macrophages in the crosstalk between exercise and immunity

在小鼠巨噬细胞系RAW 264细胞中过表达Rev-erb抑制了LPS内毒素攻击后Ccl 2的诱导，导致细胞粘附和迁移活性受损。此外，Rev-erb直接通过Ccl 2启动子区域中的Rev-erb结合基序抑制Ccl 2表达。这些发现表明Rev-erb通过直接调节Ccl 2表达来调节巨噬细胞的炎症功能。

Overexpression of Rev-erb in murine macrophage cell line RAW264 cells suppressed the induction of Ccl2 following an LPS endotoxin challenge, resulting in the impaired activity of cell adhesion and migration. In addition, Rev-erb represses Ccl2 expression directly through a Rev-erb binding motif in the Ccl2 promoter region. These findings demonstrate that Rev-erb modulates the inflammatory function of macrophages through the direct regulation of Ccl2 expression.