Study on growth and differentiation of human eosinophils

用佛波酯（TPA）和N-甲酰肽（fMLP）刺激人嗜酸性粒细胞系EoL-1，用鲁米诺依赖的化学发光法研究其呼吸爆发。用干扰素-（IFN-）处理EoL-1 5天强烈增强TPA诱导的CL应答。IFN-α也有类似的作用。肿瘤坏死因子（TNF）也能增强TPA诱导的EoL-1细胞的CL反应，并对EoL-1细胞的生长有明显的抑制作用。另一方面，白细胞介素5（IL-5）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）对EoL-1中TPA诱导的CL没有增强作用。而IL-5对IFN-γ的作用稍有增强。此外，IL-5和GM-CSF均对EoL-1显示轻微的生长促进作用。用IFN-α、-A或TNF预处理的EoL-1也能产生对fMLP的CL反应，IFN-α和TNF又有协同作用。IFN-γ增强了TNF的这种细胞学效应。进一步证明，用IFN-γ和TNF同时处理EoL-1强烈增加了佛波醇二酯的结合位点，并且还显著诱导了fMLP受体的表面表达。两者合计，EoL-1似乎提供了一个有价值的实验模型的个体发育和人类嗜酸性粒细胞的功能的研究，并作为其产品的来源。

Human eosinophilic cell line EoL-1 was studied by using luminol-dependent chemiluminescence for respiratory burst upon stimulation with phorbol ester (TPA) and N-formylpeptide (fMLP). Treatment of EoL-1 with interferon- (IFN- ) for 5 days strongly enhanced TPA-inducible CL response. IFN- A had a similar effect. Tumor necrosis factor (TNF) also enhanced TPAinducible CL response of EOL-1 and, furthermore, was quite inhiditory to EoL-1 cell growth. On the other hand, interleukin 5 (IL-5) or granulocyte-macrophage colony stimulating factor (GM-CSF) had no augmenting effect on TPA-inducible CL in EoL-1. IL-5, however, slightly enhanced the effect of IFN- . Furthermore, both IL-5 and GM-CSF showed slight growth-promoting effects on EoL-1. EoL-1 pretreated with IFN- , - A or TNf also became capable of producing CL response to fMLP, and IFN- and TNF were again synergistic.EoL-1 treated with IFN- , - A, or TNF had abundant cytoplasm, but only TNF increased cells having distinct eosinophilic granules. IFN- enhanced this cytological effect of TNF. It was further demonstrated that the simultaneous treatment of EoL-1 with IFN- and TNF strongly increased the binding sites for phorbol diesters and also dramatically induced the surface expression of fMLP receptors. Taken together, EoL-1 seems to provide a valuable experimental model for the study of the ontogeny and the function of human eosinophils and as a source of their products.