Light dosimetry of liver tissues and hepatic tumors in rats and human. --Experimental study and its application--

在410、630和670 nm波长测量了大鼠和人的肝脏和肝脏肿瘤中的光衰减。将光纤连接到光子计数系统以确定光通量，并将其连接到卡尺上以测量纤维分离的距离。手术切除后立即使用5例来自正常肝脏的转移性结肠癌和伴有肝硬变的肝细胞癌标本。转移性肿瘤在所有波长的穿透深度最深。癌组织与非癌组织间差异有统计学意义(p0.01)。此外，与正常肝组织相比，肝硬变肝组织在所有波长下均表现出显著的深度穿透(p0.01)。各种肝病大鼠肝组织的穿透深度与人切除的肝组织和肿瘤的穿透深度大体一致。肝组织和肿瘤的光剂量学在临床应用和肝肿瘤的光动力治疗中显示出区分癌组织和非癌组织的潜力。在恶性肿瘤的光动力治疗中，建议使用670 nm的光。

The attenuation of light in the liver and hepatic tumors of rats and human was measured in the 410, 630, and 670 nm wavelengths. Optical fiders coupled to a photon counting system were positioned within the tissues to determine light flux, and were attached to a caliper gauge to measure the distance of the fiber separation.Five resected specimens of metastatic carcinoma from the colon with normal liver, and hepatocellular carcinoma with cirrhosis of the liver were used immediately after surgical resection. Metastatic tumors showed the deepest penetration at all wavelenhths. A significant difference appeared between the cancerous and noncancerous tissues (p 0.01). Additionally, the cirrhotic liver tissues showed significant deep penetration by all wavelengtsexamined in comparison with normal liver tissues (p 0.01). The penetration depth of rat liver tissues with a veriety of hepatic diseases showed grossly the same tendency with those of the human resected hepatic tisues and tumors.Light dosimetry of hepatic tissues and tumors showed a potentiality of differentiation between cancerous and noncancerous tissues in its clinical application and photodynamic therapy of the hepatic tumors. In the photodynamic therapy of malignancies, 670 nm light shou ld be recommended.