Classification of Phosphate Removal Bacteria and Improvement of Phosphate Removal in the Anaerobic-Aerobic Activated Sludge Process

厌氧-好氧活性污泥法中的强化生物除磷是一种非常节约资源和能源的去除污水等废水中磷酸盐的方法。从以往的研究来看，厌氧吸收乙酸、丙酸等有机物被认为是聚磷菌的关键机制之一。本研究对厌氧-好氧活性污泥中的优势菌群植物群进行了分类，并对有机物厌氧吸收机制的最终产物进行了鉴定。主要结果如下：（1）在强化除磷工艺中，经API 20 E系统鉴定，醋酸钙不动杆菌lwoffi变种占总菌株的18%~ 50%。(2)In厌氧乙酸吸收，根据生物质中碳水化合物的降解，将乙酸转化为储存在生物质中的PHB。(3)In在丙酸吸收的情况下，在生物质中形成积累的PHB和PHV。(4)A丙酸转化为PHV的概念代谢途径被很好地定量假设。（5）PHB和PHV是可生物降解的塑料。

The enhanced biological phosphate removal in the anaerobic-aerobic activated sludge process is a very resources and energy saving method to remove phsphate from wastewater like sewage. From the previous researches, the anaerobic uptake of the organic substances like acetic acid, propionic acid, and other organics is thought to be one of the key mechanisms of the phosphate accumulating bacteria. In the present research, experimental investigations are carried out to classify the dominant bacterial flora in the anaerobic-aerobic activated sludge, and to identify the end products of the anaerobic uptake mechanisms of the organic substances. The important results are summarized as follows:(1)In the enhanced phosphate removal process, 18 to 50 per cent of total isolates were identified as Acinetobacter calcoaceticus var.lwoffi by the API 20E system.(2)In the anaerobic acetate uptake, the acetate was converted into the PHB stored in the biomass in accordance with the degradation of carbohydrate in the biomass.(3)In case of the propionic acid uptake, both PHB and PHV were formed to be accumulated in the biomass.(4)A conceptual metabolic pathway of the propionic acid conversion into PHV was postulated well quantitatively.(5)PHB and PHV are biodegradable plastics.