Studied on The Immunopathogenesis of Summer-Type Hypersensitivity Pneumonitis

为了探讨日本夏型过敏性肺炎(SHP)的免疫发病机制，我们评价了引起该疾病的主要病原体--皮肤毛孢子菌的免疫致病特性。皮肤毛滴虫培养滤液抗原经DEAE-纤维素柱层析后，得到A和B两个多糖峰。组分B对致敏兔血清和细支气管液标本中的特异性免疫球蛋白抗体和免疫球蛋白A具有较强的反应性，而组份A主要与抗体反应。当这些抗原组分经气管内注射致敏兔时，B组分可引起肉芽肿性肺泡炎，而A组分可引起肺泡炎而不是肉芽肿。根据增殖反应评估，纵隔淋巴结的淋巴细胞对组分B有显著的反应，但对组分A的反应较少。每个分数…两株不同血清型的S B在酶联免疫吸附试验中对同种抗原致敏的兔血清有较高的反应性，而对异种抗原致敏的兔血清的反应较弱，即组分B的抗原特异性与皮肤毛滴虫的血清型有关。经SepharoseCL-4B凝胶过滤，组分B的抗原组分的相对分子质量大于1,000,000，主要由多糖组成。在免疫扩散试验中，I型和II型血清的高分子量组分与同种抗血清反应呈沉淀线，而与异种抗血清无反应。这种抗原性对过氧钠敏感，对链霉蛋白酶不敏感，表明这是一种多糖性。血清相关多糖抗原吸入激发试验在SHP患者中呈阳性，但在暴露于无症状家庭成员中则不呈阳性。较少

To investigate the immunopathogenesis of Japanese summer-type hypersensitivity pneumonitis (SHP), we evaluated the immunopathogenic properties of Trichosporon cutaneum, a major causative agent of the disease. When the culture filtrate antigen of T. cutaneum was chromatographed on DEAE-cellulose, two peaks of polysaccharide, fraction A and B, were obtained. Fraction B was highly reactive to the specific IgG and IgA antibodies in the serum and bronchiol alveolar fluid samples obtained from sensitized rabbits, where fraction A was mainly reactive to the IgG antibodies. When the rabbits sensitized by the intratracheal injection with the particulate anten of T. cutaneum were challenged intratracheally by these antigenic fractions, fraction B induced granulomatous alveolitis, but fraction A induced alveolitis rather than granuloma. Lymphocytes of the mediastinal lymph node responded prominently to fraction B, but less to fraction A, as assessed by proliferative response. Each of the fraction … More s B obtained from two strains of different serotypes was reactive in enzyme-linked immunosorbent assay to the serum samples from the rabbits sensitized with homologous antigen, but far less to that from the rabbits sensitized with heterologous antigen, that is, the antigenic specificity of fraction B was related to the serotype of T. cutaneum. Fraction by gel filtration on Sepharose CL-4B revealed that the molecular weight of the antigenic components in fraction B was larger than 1,000,000 and that the components consisted mainly of polysaccharide. In immunodiffusion, the high molecular weight fractions of serotypes I and II showed precipitin lines reacting with each of the homologous antisera, but no line with the heterologous antisera. This antigenic activity was sodium-perodate-sensitive and pronase insensitive, suggesting a polysaccharide nature. Inhalation challenge test with the serotype-related polysaccharide antigen resulted in positive in SHP patients, but not in exposed asymptomatic family members. Less