NMR Studies of Protein-Anesthetic Interactions-related with anesthetic mechanism

1. 为了研究疏水水合作用，采用19F核磁共振波谱法测定了甲氧基氟烷、氟烷、安氟烷和异氟烷对水的溶解度。指出了传统溶解度法存在的问题。建立了测定挥发性麻醉剂溶解度的新方法。该研究结果已被一家国际杂志接受并发表。应用上述方法测定了挥发性麻醉剂溶解度的温度变化。从疏水水化的角度分析了挥发性麻醉剂对水结构的贡献。计算了溶解过程中冰山形成的焓变和焓变，并与烃类和全化烃的焓变进行了比较。研究结果正准备提交给一家国际期刊。为了麻醉机理的研究，公布了甲氧基氟烷、氟烷、安氟烷和异氟烷在15、20、25、30、37℃下对水的溶解度。研究了蛋白质与挥发性麻醉剂的相互作用，首次发现了挥发性麻醉剂对α -凝乳胰蛋白酶特异性活性的激活作用。机理解释为麻醉剂通过疏水相互作用促进酰基离去基部分疏水脱水。这些成果被一家国际杂志接受并发表。为了研究水合水分子对挥发性麻醉剂的动力学结构，即2中所研究的动力学结构，研究了水分子对醇的配位运动。结果表明，该运动与溶质-溶剂相互作用有良好的关系。研究结果发表在一家国际期刊上。为研究疏水水合反应的动态结构与麻醉压力逆转的关系，建立了高压核磁共振实验池并投入使用。

1. In order to study hydrophobic hydration, we measured solbilities for water of methoxyflurane, Halothane, enflurane and isoflurane by 19F NMR spectroscopy. The problems associated with traditional method of solubilities was pointed out. and we established new methodology of measuring volatlie anesthetic solubility. The results are accepted by an international journal and in printing.2. Temperature change of the volatile anesthetic solubility was measured byb applying above methodology. Volatile anesthetic contribution to water structure was analized thermodynamiceally from the view point of hydrophobic hydration. Entholopy change and enthalpy change of iceberg formation associated with dissolvment was calculated and compared with that of hydrocarbon and hologenated hydrocarbon. The results is preparing for submission to an international journal.3. For the use of investigators of anesthetic mechanism, solubilities for water of methoxyflurane, halothane, enflurane and isoflurane in 15, 20, 25, 30, 37 C were published.4. Protein-volatile anesthetic interaction was investigated, and activation by volatile anesthetic of specific activity of alpha-chymotrypsin was discovered for the first time in the world. The mechanism was explained that the anesthetics promoted partial hydrophobic dehydration of acyl leaving group of through hydrophobic inter actions. The fruits was are accepted and in printing by an international journal.5. In order to investigate dynamic sturucture of hydrated water molecule to volatile anesthetics, which is the rmodynamically investigated in 2, molecular motion of water molecule coordinated to alcohol was investigated. The motion turns out to have good relationship with solute-solvent interactions. The results is submitted to an international journal.6. To study the relation between dynamic structure of hydrophobic hydration and the pressure reversal of aneslhesia, high pressure NMR cell was constructed and is now working.