Study on the novel bioactive substances in follicular fluid regulating a oocyte maturation and its development.

1.本文用放射免疫法测定了18例超促排卵妇女的血清卵泡素水平，以评价卵泡发育。卵泡早期血清胆红素水平与hCG日卵泡直径<greater than or equal>17 mm（r=0.57）、<greater than or equal>14 mm（r=0.67）相关。这些数据表明：在早期卵泡期，血清β-D-半胱氨酸可能是预测卵泡发育的有效指标;在排卵前期，血清β-D-半胱氨酸可能是预测超排卵期卵泡发育的有效指标之一。激活素A对PMSG处理的未成熟大鼠卵母细胞减数分裂成熟的影响进行了分析;激活素A作为红系分化因子被纯化，不仅加速卵泡封闭的卵母细胞和卵母细胞丘复合体的成熟，而且加速裸露的卵母细胞的成熟，通过增加具有生殖囊泡破裂（GVBD）的卵母细胞的百分比来测量。在GVBD抑制剂cyclic-AMP存在下，激活素A不能促进卵母细胞成熟，表明激活素A是一种有效的卵母细胞体外成熟刺激剂.在1-3天的时间内，每天皮下注射40 μ g激活素A至完整的未成熟雌性大鼠，引起血清FSH、雌二醇、子宫重量和卵巢FSH受体的显著增加。每天皮下注射5 μ g或20 μ g激活素A，连续6天，可使卵巢重量明显增加，并产生大卵泡。同时注射激活素A和5IU PMSG可显著增加卵巢和子宫重量，并可显著增加血清雌二醇和雌二醇水平。与在切除垂体的未成熟雌性大鼠中同时注射溶媒和PMSG相比，

1. In order to appraise follicular development, human serum inhibin was measured daily by radioimmunoassay in 18 women undergoing ovarian hyperstimulation. Serum inhibin in the early follicula phase correlated with the number of follicles<greater than or equal> 17 mm in diameter(r=0.57), follicles<greater than or equal>14 mm in diameter(r=0.67)on the day of hCG administration. These deta suggest that : In early follicular phase, serum inhibin may be a valid index to predict ensuing follicular growth ; In the preovulatory Phase, serum inhibin may by one of applicable indexes of follicular development during the hyper2. The effect of activin A on meiotic maturation was analyzed in oocyte from imroature rats treated with PMSG ; Activin A, which was purified as the erythroid differentiation factor, accelerated the maturation of not only follicle-enclosed oocytes and oocytecumulus complexes, but also denuded oocytes, as measured by an increase in the percentage of oocytes with germinal vesicle breakdown(GVBD). Oocyte maturation was not accelerated by activin A in the presence of the inhibitor of GVBD such as cyclic-AMP, These results showed activin A is a potent in vitro stimulator of oocyte maturation.3. Daily sc injections of 40 ug activin A over a period of 1-3 days to intact immature female rats caused a significant increase in serum FSH, inhibin, estradiol, uterine weight and ovarian FSH receptors. Daily sc injections of 5 ug or 20 ug activin-A for 6days caused a marked increase in ovarian weight and the development of large ovarian follicles. Simultaneous injections of both activin-A and 5IU PMSG induced a significant increase in ovarian and uterine weight, serum inhibin and e. stradiol levels, compared to simultaneous injections of both vehicle and PMSG in the hypophysectomized immature female rats.