Studies on the evaluation of photo allergic skin lesions from pesticides

为了评价农药引起的光致过敏性皮肤损害，进行了案例分析和实验研究。实验结果如下：1.实验结果表明：从农药致皮损病例分析来看：在报告系统收集的农药致皮损病例中，疑似光过敏的发生率相对较低，约占总数的2%。致病性农药多种多样，有机磷杀虫剂和硫类杀菌剂相对占优势。但确诊为光致过敏性接触性皮炎的病例数量较少。从农药光致过敏皮肤损害的实验研究来看：(1)激光多普勒血流法（LDF）评价反应；以豚鼠和农药为队长，PNCB和zineb为实验模型，对LDF法与裸眼读数的评价进行了比较研究。从方法上看，与裸…More评价不同的是，紫外辐照PNCB高、低剂量组在24、48 h评价时其流量表现出明显的高反应，24 h评价时船长高剂量组也表现出明显的高反应。用该方法估计了某些农药之间的交叉反应，并推测该方法可用于光敏性评价。但是，从该方法中，很难区分由肉眼读数估计的阴性反应和假阳性反应。(2)条带辅助技术评价农药光敏性；根据我们以往的经验和大量的参考文献，选取了zineb、captain、PNCB、DDVP、百菌清、百草枯等农药，对其光敏性进行了评价。其中百菌清对光致敏反应表现出中高效，PNCB和百草枯对光致敏反应也表现出轻度效。但以DDVP为例，他们的实验结果在阅读上存在一些差异。结果表明，LDF法可用于光致敏性皮肤损伤的评价，部分农药具有光敏性。少

In order to evaluate photo allergic skin lesions from pesticides, case analyses and experimental studies were undertaken. The results were as follows :1. From case analyses of skin lesions from pesticides : In cases of skin lesions from pesticides collected by reporting system, the incidence rates suspected to be photo allergy were relatively lower, i. e., about 2% in total number. The causative pesticides were various, and organophosphorus insecticides and sulfur fungicides were relatively dominant. But, the cases diagnosed definitely as photo allergic contact dernatitis were only small in number.2. From experimental studies on the skin lesions of photo allergy from pesticides :(1)Evaluation of the reaction using laser-Doppler flowmetry(LDF)method ; Comparative studies on the evaluation between the LDF method and the reading with the naked eyes were undertaken using guinea pigs and pesticides as captain, PNCB and zineb as a experimental model. From the method, different from the naked … More evaluation, high and low dose groups with PNCB irradiated to ultraviolet showed significantly high reaction at 24 and 48 hrs evaluation in its flow volume, and also in captain high dose group at 24 hr evaluation. The cross reactions among some pesticides were also estimated from the method, and it was suspected that this method is useful to evaluate photo sensitivity. But, from the method, it was difficult to distinguish between negative reaction and pseudopositive reaction estimated by the reading of the naked eyes.(2)Evaluation of photo sensitivity from pesticides by the Strip-Adjuvant technique ; am pesticides as zineb, captain, PNCB, DDVP, chlorothalonil, paraquat, etc. selected by our past experience and many references were studied to evaluate their photo sensitivity. Among those pesticides, chlorothalonil showed moderate to high potency of photo allergic reaction, and PNCB and paraquat also showed mild potency of photo allergic reaction. But, in case of DDVP, there were some discrepancies in reading on their experimental results. From the results, it was concluded that LDF method is useful to evaluate photo allergic skin lesions and some pesticides were also estimated to have a potency to cause photo sensitivity. Less