Studies on roles of mucosal mast cells in protection against intestinal parasites

重复的IP地址。注射IL-3可诱导C57BL/6小鼠的肥大细胞增多症，是Ratti(Sr)感染小鼠肥大细胞增多率峰值的30-40%。这一发现可以促进对粘膜肥大细胞(MMC)的研究。锶的排出由两个步骤引起，即从小肠到盲肠和从盲肠到外面。虽然sr感染使盲肠中的MMC数量增加，但盲肠中的嗜酸性粒细胞和杯状细胞数量也显著增加，提示盲肠和小肠之间存在不同的作用机制。当裸小鼠同时感染锶和巴西新城疫杆菌(Nb)并反复注射IL-3时，锶被排出，而Nb不被排出。因此，锶和Nb的排泄机制是不同的。C57BL/6小鼠反复注射IL-3总量超过10^4U后再感染sr，对肠道蠕虫有较强的保护作用。有效的Protec…的诱导再注射IL-3需4~5天。其保护作用与肠道MMC数量的增加密切相关。小鼠注射IL-3后口服sR后，在感染后6h内开始驱虫。因此，IL-3诱导的保护作用与抗原特异性免疫反应无关。注射IL-3不仅对经口感染的幼虫有保护作用，对成虫也有保护作用。然而，对S.C.后从头部恢复的组织移行幼虫没有诱导保护作用。感染。IL-3不能诱导肥大细胞缺陷W/W、V小鼠对锶的保护作用。IL-3注射使未感染小鼠肠道MMCP-1含量和肠道MMC数量增加200倍。因此，MMC可以在没有IgE抗体的情况下引起脱颗粒。所有这些结果表明，MMC对锶的保护作用与肠道保护有关，而对NB的保护作用不明显。不同种类线虫的驱虫作用机制不同。较少

A repetitive i.p. injection of IL-3 induces mastocytosis which is 30-40% of the peak mastocytosis in Strongyloides ratti (Sr)-infection in C57BL/6 mice. Studies on mucosal mast cells (MMC) can be progressed by this finding. Expulsion of Sr was caused by two steps, from the small intestine to the caecum and from the caecum to outside. Although number of MMC in the caecum was increased by Sr-infection, numbers of eosinophils and goblet cells were also increased markedly in the caecum, suggesting a different effector mechanism is operating between the caecum and the small intestine. When nude mice were concurrently infected with Sr and N. brasiliensis (Nb) and injected repeatedly with IL-3, Sr was expelled but Nb was not. Therefore, mechanism of expulsion is distinct between Sr and Nb-infection. C57BL/6 mice injected repeatedly with more than a total of 10^4 U IL-3 and then infected with Sr showed strong protection against the worm in the small intestine. Induction of the effective protec … More tion by IL-3 injection took 4 to 5 days. The protective effect was well correlated with number of intestinal MMC increased. Mice injected with IL-3 and then orally infected with Sr began to eliminate the worms within 6 h post-infection. Therefore, the protection induced by IL-3 should have no concern with antigen specific immune responses. IL-3 injection could induce protection against not only larvae but also adult worms infected orally. However, it was ineffective to induce protection against tissue migrating larvae recovered from the head after s.c. infection. IL-3 injection could not induce protection against Sr in the mast cell deficient W/W^V mice. IL-3 injection increased amount of MMCP-1, a protease specific to MMC, in the intestinal lumen by 200 times and number of intestinal MMC in uninfected mice. MMC, therefore, can cause degranulation without IgE antibodies. All these results indicate that MMC are concerned with intestinal protection against Sr but not against Nb. Effector mechanisms of the worm expulsion should be different among various species of nematodes. Less