Experimental study of lymph node metastasis on oral cancer

从转移到仓鼠下颌下淋巴结的鳞状细胞癌（O-1 N）中建立了具有高转移潜能的区域淋巴结细胞系。O-1 N是将化学诱导的舌鳞状细胞癌的颌下淋巴结转移瘤连续移植到金黄地鼠的贮袋内建立的。在单层片上持续生长的培养细胞已在35个月内保持72代。将第7代和第18代细胞分别接种到10只和5只仓鼠的颊囊中，导致所有动物均产生颊部肿瘤伴淋巴结转移。为了检测器官-组织源性因子对O-1-N存活和生长的影响以及淋巴结定植特性，我们用仓鼠组织：肺和淋巴结来检测器官-组织源性因子对O-1-N存活和生长的影响。制备器官组织块，用O-1 N培养低血清浓度的条件培养基，O-1 N细胞重新接种于低血清浓度的培养基中，只有极少量的生长。加入器官条件培养基对O-1 N细胞生长的影响最小。

A cell line with a high metastatic potency to regional lymph nodes was established from a squamous cell carcinoma(O-1N)metastatic to a submandibular lymph node of a hamster. The O-1N was established by serial trarnsplantation of a metastatic tumour to the submandibular lymph node from a chemically induced squamous cell carcinoma of the tongue into the pouch in hamster. Cultured cells which constantly grew in a monolayred sheet have been maintained through 72 passage over 35 months. Inoculation of cells of passage 7 and 18 into the buccal pouch of 10 and five hamsters, respectively, resulted in the production of buccal tumours with lymph node metastases in all animals. The findings were essentially consistent with those exhibited by O-1-N in an in vivo model.To test the effect of organ-tissue-derived factors on the survival and growth of O-1N of lymph node colonization property we used the hamster tissues : lung and lymph nodes. Organ tissue pieces were prepared, and the conditioned media from suspensions cultures of these tissues in low serum concentrations were incubated with O-1N.Replating O-1N cells in medium with low serum content allow only minimal growth. Addition of the organ-conditioned medium to O-1N cells had minimal effects on O-1N cell growth.