Studies on Specialty Biomaterials Carrying Glycochains as Biological Ricognition Signals

以碳水化合物作为细胞间相互作用的主要参与者，合成了结构明确的含碳水化合物链的高分子材料，并将其应用于细胞特异性生物医用材料。（1）以脱水糖衍生物为原料，通过开环聚合反应，合成了3-脱氧-3-氟-（1 × 6）-α-D-吡喃葡萄糖。（2）通过β-半乳糖苷酶催化合成大分子单体，再进行自由基聚合，合成了一类含N-乙酰乳糖胺衍生物的新型糖基共聚物。（3）通过磷酸化酶催化直链淀粉取代苯乙烯衍生物的合成及其聚合反应，合成了由合成聚合物主链和多糖接枝物组成的接枝共聚物。（4）通过甲基丙烯酰氧基乙基α-D-吡喃葡萄糖苷的大分子单体，获得了具有成簇葡萄糖苷的两亲性接枝共聚物。(5)A以乳糖载体聚苯乙烯为基质，通过共培养，构建了一种新型的肝细胞集合体，包括肝细胞、内皮细胞和枯否细胞。（6）红细胞倾向于与带有还原性葡萄糖部分的聚苯乙烯衍生物牢固结合。葡萄糖转运蛋白分子和还原葡萄糖基团之间的动态识别建议。

Well-defined polymers having carbohydrate chains have been synthesized and applied for cell-specific biomedical materials using the carbohydrates as the key participants in cell-cell recognitions.(1)Regiosperfcifically fluorinated stereoregular polysaccharides, 3-deoxy-3- fluoro-(1*6)-alpha-D-glucopyranan was synthesized via ring-opening polymerization of anhydro sugar derivative.(2)beta-Galactosidase-catalyzed synthesis followed by radical polymerization of macromonomer gave a new type of glycopolymers having biologically important N-acetyllactosamine derivatives.(3)Graft copolymers consiting of synthetic polymer backbone and polysaccharide grafts have been synthesized by phosphorylase-catalyzed synthesis of amylose-substituted styrene derivative and its polymerization.(4)Amphiphilic graft copolymers having clustered glucosides have been obtained via macromonomers of methacryloyloxyethel alpha-D-glucopyranoside.(5)A new type of assemblies of liver cells containing hepatocytes, endothelial cells, and Kupffer cells have been constructed by co-culture on lactosecarrying polystyrene as substratum.(6)Erythrocytes have been fond to attach strongly to a polystyrene derivative carrying reducing-glucose moieties. The dynamic recognition between glucose transporter molecules and reducing glucose moieties is suggested.