A STUDY ON SIMULTANEOUS DETERMINATION OF VOLATILE COMPOUNDS IN HUMAN TISSUES

建立了一种可靠、灵敏的含有硫化物、多硫化物、硫代硫化物、尼古丁和可替宁等挥发性物质的分析方法。在硫化物方面，进行了动物实验，用GC/ECD测定了组织中的硫化物和硫代硫化物的浓度。死亡病例宜取血、肺或脑，存活者宜取尿进行分析。在多硫化物中毒方面，用GC和GC/MS对多硫化物、硫化物及多硫化物分解产物进行了分析。从动物实验数据中检测出体内组织中的多硫化物和硫化物，证实了多硫化物中毒。简要描述了两例疑似多硫化物中毒的实际病例。从尼古丁和可替宁的角度，建立了一种可靠、灵敏的GC/MS分析方法。对10例尸检组织中尼古丁和可替宁的分布进行了测定。从实验数据来看，当采集血液不可行时，肌肉是最适合进行毒理学检查的组织。

A reliable and sensitive methods of volatile compounds containing of sulphide, polysulphide, thiosulphide, nicotine and cotinine were developed to determine what was the most suitable tissue for the toxicological analysis.With the respect of sulphide, animal experiments have been made to determine the concentrations of sulphide and thiosulphide in tissues which were measured by GC/ECD.From the data, thiosulphite was proposed for the indicator of hydrogen sulphide poisoning ; blood, lung or brain is recommendable for fatal cases, and urine for survival cases, as materials to be analyzed.With the respect of polysulphide poisoning, polysulphide, sulphide and the decomposed products of polysulphide, were analyzed by GC and GC/MS.From the animal experimental data, detecting of polysulphide and sulphide in body tissues proved the polysulphide poisoning.Two practical cases suspected poisoning by polysulphides are briefly described.With the respect of nicotine and cotinine, a reliable and sensitive method was developed using GC/MS.The distributions of nicotine and cotinine were measured in tissues from 10 autopsy cases. From the experimental data, muscle was the most suitable tissue for toxicological examination, when acquisition of blood is not feasible.