Development of a new appratus for continuous recording of mechanical activity of isolated arterioles

a .构造了一种照相机式测径仪，并用于生理实验。这种量规的设计和使用特点可以概括如下。在单镜头反射相机的焦平面上安装一个图像传感器，配备一组延伸管，可以测量小到100微米的血液和淋巴管的外径。用这个量规，我们可以分辨出直径变化约12微米。同时测量两种不同血管的直径可以使用两个窗口信号，在视频扫描中在适当的时间内独立产生。b .从狗的分布肌肉动脉中分离出纵向条，用去甲肾上腺素松弛，用罂粟碱收缩。这种矛盾的松弛和收缩可能是由于平滑肌在壁上的螺旋和圆形排列是不可压缩的。各种血管收缩剂，包括norad和More肾素，在肌肉动脉中产生圆周收缩和纵向松弛。血管扩张剂产生相反的效果。这种矛盾的反应使我们在小动脉的药理研究中找到了一种新的方法。利用这种方法，我们成功地研究了外径0.3 ~ 0.5 mm的小动脉的药理反应。c .我们构建了一种新的仪器，用于连续记录外径0.05 ~ 0.15 mm的离体小动脉的机械活性。使用杜林和里弗斯（1986）的方法剥离细动脉的许多组织。然后将血管段转移到倒置显微镜台上的1 ml温控室中，在玻璃吸管两端插管，加热至37℃。小动脉两端的移液器连接到水压计，将腔内压力设置为40 mmHg，以匹配先前报道的体内压力。在x320放大镜下观察小动脉，用摄像机记录其图像，并在电视显示器上显示。用视频卡尺和微型计算机连续测量血管直径、壁厚、内外压力和壁张力。少

A.A camera-type diameter gauge was constructed and used in physiological experiments. The distinctive features of the design and use of the gauge may be summarized as follows. Installation of an image sensor in the focal plane of a single-lens reflex camera equipped with a set of extension tubes which makes it possible to measure outer diameters of blood and lymph vessels as small as 100 micrometer.Using the gauge, we can distinguish a diameter change of about 12 micrometer. Simultaneous measurements of the diameters of two different vessels can be made using two window signals generated independently at desired moments for appropriate periods within the video scans.B.Longitudinal strips isolated from dog distributing muscular arteries were relaxd by noradrenaline and contracted by papaverine. This paradoxical relaxation and contraction may be due to a helical and circular arrangement of smooth muscle in the wall which is incompressible. Various vasoconstrictive agents, including norad … More renaline, produced a circumferential contraction and longitudinal relaxation in muscular arteries. Vasodilator agents produced the opposite effect. The paradoxical responses led us to a new method in pharmacological research of small arteries. By the use of this method, we have successfully studied pharmacological responses of small arteries ranging from 0.3 to 0.5 mm in outer diameter.C.We have constructed a new appratus for continuous recording of mechanical activity of isolated arterioles ranging 0.05 to 0.15 mm in outer diameter. An arteriole was dissected free of many tissues using the method of Duling and Rivers (1986) . The vessel segment was then transferred to a 1-ml temperature-controlled chamber on the stage of an inverted microscope, cannulated at both ends of glass pipettes, and heated to 37ﾟC.The pipettes at each end of the arteriole were connected to water manometers, and the intraluminal pressure was set at 40 mmHg to match previously reported in vivo pressure. The arteriole was observed at x320 magnification, and its image was recorded using a video camera and displayd on a television monitor. Vessel diameters, wall thickness, inner and outer pressures, and wall tension were continuously mesurements with a video caliper amd microcomputor. Less