Improvement of Enrichment Culture Used Iron-oxidizing Bacteria having Inhibition to Cupper

研究了某矿山废弃物富集培养物对亚铁离子和单质硫的氧化作用。在含硫培养中，在Cu^<2+>浓度、0、1、10、100、1000mg dm^<-3>条件下，测定了细菌从S^0产生的SO^2-_的时间变化，以及细菌和原生动物的细胞数量。在亚铁离子培养中，本研究还通过一些无营养盐或SDS抑制细菌生长的铁氧化实验，探讨了与细菌的回声系统。在含亚铁离子的培养液中，利用高效液相色谱法对分离出的细菌产生的有机物进行了分析。研究结果如下：(1)原生动物使硫氧化菌的氧化速率和产率比单独培养的细菌要小；(2)细菌在Cu^<2+>的抑制下生长，而原生动物在Cu^<2+>浓度大于100mg dm^<-3>时不生长，原生动物不出现对Cu^<2+>的抗性。;(3)在缺乏营养盐的情况下，富培养中铁氧化菌的氧化速率随时间增加而增加；(4) SDS浓度在1*10^<-5>mol dm^<-3>时，铁氧化芽孢菌受到抑制；(5)用高效液相色谱法对培养液中细菌产生的有机物进行检测。

The oxidation of ferrous ion and elemantal sulfur by the enrichment culture collected from a mine waste is investigated. In the culture with sulfur, the time-changes of SO^2-_ produced by the bacteria from S^0 and the cell numbers of the bacteria and the protozoa are measured under the conditions, Cu^<2+> concentration, 0,1,10,100,1000mg dm^<-3>. In the culture with ferrous ion, this study, also, investigated an echosystem with the bacteria through some experiments of iron oxidation in absence of nutritive salts or with SDS that inhibits the growth of the bacteria. In the culture broth with ferrous ion, further, organic materials produced by the bacteria liberated were analyzed by HPCL.The results are as follow ;(1) the protozoa makes the bacterial oxidation rate and the yield factor of sulfur-oxidizing bacteria growth smaller than those in a cultivation of only the bacteria ; (2) while the bacteria grow under the inhibition by Cu^<2+>, the protozoa do not grow at Cu^<2+> concentration above 100mg dm^<-3>, and the resistence to Cu^<2+> does not appear in the protozoa. ; (3) for the absence of nutritive salts, the oxidation rate by the iron-oxidizing bacteria in enrichment culture increases with time ; (4) the iron-oxidizing bacreia is inhibited above 1*10^<-5>mol dm^<-3> in SDS concentration ; (5) organic materials produced by the bacteria are detected by HPCL in the culture broth.