Genetic studies of Dipteyocarpaceae in Malaysian rainforests

该项目的主要目标是获得植物遗传和物种多样性（统称为生物多样性）的基本数据，并设计有效保护它们的方案。我们集中研究了三个不同森林种群的遗传和物种多样性; 2泰国季风林的内陆树木，日本北海道的松树，以及马来西亚热带雨林的Shorea。3日本种群中要研究的物种是松树和日本雪松的几个物种。泰国目标植物种群包括全国各地的许多自然种群以及至少一个人造森林，即所谓的威布尔先生森林。包括龙脑香属在内的多种内陆树种，以及20多种红树植物。从不同树木的叶片样品中纯化DNA后，利用叶绿体tRNA和细胞核GAP-C基因的间隔区进行DNA测序。在泰国的四种主要内陆树种中没有发现任何遗传变异，而在日本和马来西亚种群中发现了很高的遗传变异。在日本和马来西亚人群中发现的基因多样性的数量从0.002到0.01，这取决于所研究的基因和人群。泰国季风林和其他种群（马来人和日本人）之间遗传变异的这种显著差异可能会在热带森林的维护和可持续利用方法中引起严重问题。

Our main objective of the project is to obtain basic data on genetic and species diversity, collectively called biodiversity, of plants and design schemes to preserve them efficiently. we focused at the study of genetic and species diversity in three different forest populations ; inland trees of monsoon forests in Thailand, pine trees in Hokkaido, Japan, and Shorea of Malasian tropical rain forests Species to be studied in Japanese populations are several species of pinetrees and Japanese cedar. Plant populations in target in Thailand include many natural populations available throughout the country as well as at least one man-made forest, so called Mr.Wibul forest. Many species of inland trees including Dipterocarpus group, in addition to over 20 spesies of manglobe trees are covered in this study. After purifying DNA from leaf samples of various trees DNA sequencing were done using an spacer region of tRNA of chloroplast and GAP-C genes of nucleus. No genetic variatio was found in any of dominat four species of inland trees in Thailand, in contrast to the high genetic variability found in both Japanese and Malasian populations. The amount of gene diversity found in Japanese and Malasian populations were from 0.002 to 0.01 depending on the genes and populations investigated. This striking differences in genetic variations between Thailand monsoon forests and other populations (Malasian and Japanese) may raise serious problem in the method of maintenance and sustainable use of tropical forests.