Monoclonal Antibody Recognizing Peroxidized Lipoproteins Accumulated in Atherosclerotic Lesion.

本研究阐明了脂质过氧化与动脉粥样硬化病变的关系。低密度脂蛋白的氧化修饰被认为与动脉粥样硬化的发生和促进有关。它们被巨噬细胞和平滑肌细胞吸收，导致胆固醇酯的大量积累。近年来，我们研制了一种新的单克隆抗体（FOH 1a/DLH 3），它识别氧化型低密度脂蛋白，而不识别MDA-LDL和乙酰化型低密度脂蛋白。抗体的表位可以是氧化磷脂酰胆碱（9 CHO-PC），其中一些可以与多肽形成复合物。

In this research work, an involvement of lipid peroxide in atherosclerotic lesions was elucidated Oxidative modiffication of low density lipoproteins are believed to be involved in the intiation and promotion of atherosclerosis. They are taken up by macrophages and smooth muscle cells resulting in a massive accumulation of cholesteryl ester. Recently we developed novel monoclonal antibody (FOH1a/DLH3) which recognizes oxidized LDL but MDA-LDL,acetylated LDL.Immunohistochemical analysis in human coronary arteries showed that foam cells derived from macrophage in atherosclerotic lesions heavily stained by the antibody. Epitope of the antibody may be oxidized phosphatidylcholine (9CHO-PC), some of which may form complex with polypeptides.