Reconstruction of periodontal tissues by BMP combined with functional fibrous collagen membrane

我们已经建立了利用骨形态发生蛋白（BMP）和各种载体的异位软骨和成骨系统。我们发现BMP诱导的细胞分化高度依赖于这些载体的性质。这些BMP载体分为三种类型：纤维型、颗粒型和块状。(1)纤维型包括纤维胶原膜（FCM）和纤维玻璃膜（FGM）。(2)粒子类型：胶原珠、多孔羟基磷灰石颗粒（PPHAP）和羟基磷灰石固体颗粒;（3）块状：多孔羟基磷灰石块和蜂窝状羟基磷灰石。结果表明，以FCM为载体的BMP-2和S300 BMP具有较强的协同作用，最低有效量的BMP混合物（30 μ g）与10 mg胶原微球复合后，其半数诱导骨形成的效果不明显。另外，将1.2ug rhBMP-2（由Yamanouchi Co.没有诱发可检测到的骨。然而，将20 μ g BMP混合物、1.2 μ g rhBMP 2和10 mg胶原珠组合，在植入后2周观察到显著的骨形成（每个植入物2 IU ALP和3.5 mg钙）。结果表明，BMP混合物中的某些组分与rhBMP-2具有有效的协同作用。以上结果为BMP在牙周组织再生中的临床应用开辟了一条新的途径。

We have previously establish a system of ectopic chondrogenesis and osteogenesis by using bone morphogenetic proteins (BMPs) and various kind of carriers. We found that BMP-induced cell-differentiation is highly dependent on the nature of these carriers. These BMP-carriers were classified into three types : fiber-, particle-and block-types. (1) The fiber-type includes fibrous collagen membrane (FCM) and fibrous glass membrane (FGM). (2) The particle-type : collagen beads, porous particles of hydroxyapatite (PPHAP) and solid particles of hydroxyapatite, and (3) the block-type : porous blocks of hydroxyapatite and honeycomb-shaped hydroxyapatite. By FCM carrier, we could regenerate not only alveolar bone but also cementum and periodontal membrane in cat. Furthermore, we found a strong synergistic effect between rhBMP-2 and S300 BMP.Half of the minimum effective amount of the BMPs cocktail (30 ug) which was combined with 10 mg of collagen beads as a carrier did not induce detectable bone formation. Also, 1.2 ug of rhBMP-2 (gifted from Yamanouchi Co.) did not induce detectable bone. However, 20 ug of BMPs cocktail, 1.2 ug of rhBMP2 and 10 mg of collagen beads were combined, prominent bone formation (2 IU of ALP and 3.5 mg of calcium per implant) were observed at 2 weeks after implantation. The results indicated that certain component (s) in the BMPs cocktail possessed a potent synergistic effect with rhBMP-2. Above results will open a new avenue of clinical application of BMP for periodontal regeneration.