Studies on Regulation of Macrophages Function during Nidation-Molecular and Cell Biological Studies-)

哺乳动物胚胎的体外操作，包括外源基因的引入，在许多情况下，只有在成功的胚胎移植导致新生儿出生之后才被认为是完整的。然而，已经充分认识到，胚胎移植的成功率很低，植入过程是众所周知的瓶颈。为了防止着床失败，阐明着床的细胞或分子过程的机制将是必不可少的。本项以日两国合作项目的主要目的是获得以色列在巨噬细胞研究和转基因山羊生产方面的专业知识和信息。我们尝试建立滋养层细胞体外培养细胞系，结果表明，体外培养的柴坝山羊子宫内膜上皮细胞和巨噬细胞在体外对子宫内膜上皮细胞和巨噬细胞的增殖具有协同作用。文化系统，这将作为一个模型来分析 ...更多信息 巨噬细胞对着床和随后的着床过程的影响。虽然山羊腔上皮细胞尚未克隆，但我们可以建立其培养细胞系。山羊腔上皮细胞在体外形成腺体样结构，部分细胞分化为分泌细胞，细胞质中充满分泌颗粒。实验正在进行中，以克隆这些上皮细胞系。滋养层细胞培养物遭受的突然下降，增殖能力，经过几代。人们正在尝试恢复假定的滋养层细胞系的增殖能力。结果表明，无论是从骨髓还是从脾中获得芝山羊的巨噬细胞都很困难。由于一些未知的原因，山羊巨噬细胞似乎表现出与小鼠细胞不同的行为。需要进一步的实验来建立收集和培养山羊巨噬细胞的方法。在以色列方面，目前正在进行对巨噬细胞激活过程中起作用的信号转导系统的生化分析。交流有关巨噬细胞实验和转基因山羊生产实验的最新宝贵信息，MRI方法在小鼠卵母细胞着床研究和体外成熟研究中的应用。少

The in vitro manipulation of mammalian embryos, including the introduction of exogenous genes, will be considered to be complete, in many instances, only after the successful embryo transfer leading to the birth of newborns. It has been well recognized, however, that the enbryo transfer suffers from poor success rates, the implantation processes being the notorious bottle neck. In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular processes of implantation will be essential. The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and macrophages of Shiba goats in order to work out the in vitro co-culture systems which will serve as a model to analyze … More the influence of macrophages upon the implantation and subsequent placetation processes. We could establish the cultured cell lines of caprine luminal epithelial cells, although they have not yet been cloned. The caprine luminal epithelial cells undergo formation of gland-like structures in vitro, and some of the cells were seen to differentiate into secretory cells possessing full of secretory granules in the cytoplasm. Experiments are underway to clone these epithelial cell lines.Trophoblast cell cultures suffered from the sudden reduction in the proliferative capacities, after passages of several passages. attempts are being made to restore the proliferative capacities of the putative trophoblast cell lines.Macrophages of Shiba goats turned out to be difficult to obtain either from the bone marrow or from the spleen. Owing to some unknown reasons, the caprine macrophages appear to behave differently from the murine cells. Further experiments are needed to establish methodology for the collection and culture of the caprine macrophages.On Israeli side, biochemical analysis of the signal transduction systems which operate during the activation of macrophages in currently in progress.Exchange of valuable up-to-date information concerning the experiments in not only macrophages but also production of transgenic goats, possible application of MRI methods in implantation reserach and in vitro maturation of murine oocytes, were done. Less