Molecular biochemical study on pathogenesis, especially toxin and adhesive factor, of Vibrio parahaemolyticus

本研究旨在探讨副溶血性弧菌的发病机制。实验结果如下：热稳定性直接溶血素（TDH）和TDH相关溶血素（TRH）的研究：我们发现TDH通过其n端区识别特定受体，然后诱导25 kDa蛋白磷酸化，导致溶血。我们还发现TDH、TRH和脲酶基因紧密相连，并存在于50kb片段内（一个致病岛？）。2。粘附因子的研究：我们发现cHA（细胞相关血凝素）是副溶血性弧菌的粘附因子。cHA是由4个26kDa亚基组成的四聚体蛋白，cHA的受体是一个分子量为17kda的糖缀合物。其他结果：我们还证明了副溶血性弧菌在体外培养细胞系统中的侵袭性，这可能解释了由该有机体引起的败血症的原因。对TDH、TRH或两者同时检测的方法进行了研究和评价，发现了一种新的TDH变异。

The purpose of this research is to investigate detailed pathogenesis of Vibrio parahaemolyticus. Following results were obtained.1. Studies on thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) : We found that TDH recognized a specific receptor through its N-teminal region and then induced phosphorylation of a 25 kDa protein, resulting in hemolysis. We also found that genes for TDH,TRH and urease are closely linked and present within 50kb-fragment (a pathogenicity island? ).2. Studies on adhesive factors : We identified cHA (cell-associated hemagglutinin) as an adhesive factor of V.parahaemolyticus. The cHA is a tetrameric protein consisting of 4 subunit of 26kDa and the receptor for cHA is a glycoconjugate having molecular weight of 17kDa.3. Other results : We also demonstrated the invasive character of V.parahaemolyticus in vitro cultured cell system, which may explain the cause of septicemia due to this organism. The methods detecting TDH,TRH or both were developed and evaluated, and a new variant of TDH was found.