DEVELOPEMENT OF ASSAY SYSTEMS FOR EARLY DIAGNOSIS AND DETECTION OF CHLAMYDIAL INFECTIONS

我们开发了在时间、灵敏度、特异性和操作上具有优势的衣原体感染的早期诊断和检测所需的检测系统。我们建立了鹦鹉热衣原体、肺炎衣原体和沙眼衣原体特异性的聚合酶链反应（PCR）作为基因诊断系统。我们将间接免疫荧光法应用于猫衣原体病，揭示了猫衣原体病在日本的广泛传播。此外，使用几种猫衣原体菌株，猫衣原体的抗原多样性。因此，本研究结果表明，日本的猫中存在可通过遗传学鉴别的猫衣原体感染，本文综述了衣原体感染的分子诊断方法的优缺点，建立了衣原体感染的分子诊断方法。这些方法使鸟类的“活体”诊断成为可能，并可应用于各种鸟类的筛选。我们希望这些结果能有助于衣原体感染的调控和消灭。

We developed assay systems required for early diagnosis and detection of chlamydial infections which have advantages on time, sensitivity, specificity, and handling.We established polymerase chain reaction (PCR) specific for C.psittaci, C.pneumoniae, and C.trachomatis as gene diagnostic system.Applying indirect immunofluorescence method to feline chlamydiosis, we revealed the wide spread of feline chlamydiosis in Japan. Furthermore, using several feline chlamydial strains, antigenic diversity was suggested on feline chlamydia. Therefore, the results indicated that feline chlamydia which can be distinguished genetically have spread in cats of Japan.We reviewed the advantage and disadvantage of molecular diagonositc assay for chlamydial infections.In conclusion, molecular diagnosis for chlamydial infections was established in this project. These memthods makes 'live' diagnosis of birds possible and can be applied to screening of various birds. We hope the results can contribute the regulation and extinction of chlamydial infections.