Establishment and estimation of pharmacological bioassay system for therapeutic drugs of aging in central nervous system.

为了阐明毒蕈碱型乙酰胆碱（mACh）受体亚型M1和M2在学习和记忆中的生理作用，我们研究了三种拮抗剂阿托品（阿托品）（非选择性），哌仑西平（M1选择性）和11-[[2[[（二乙基氨基）甲基]-1-哌啶基]乙酰基]-5，11-二氢-6H-吡啶并[2，3-B][1，4]苯并二氮杂卓-6-酮，AF-DX 116（M2选择性）对小鼠的stpe-through被动回避任务的影响。训练前5 min给予阿托品（1-40 nmol）和哌仑西平（10和40 nmol）可缩短获得训练后14 d的反应潜伏期。测试前（5分钟前）和训练后（收购后立即）给予阿托品轻微但不显着损害保留分数。AF-DX 116的施用对任何测试中的分数没有明显影响。因此，M1受体亚型偶联系统似乎在获取-巩固过程中比在检索过程中更重要。

To clarify the physiological roles of muscarinic acetylcholine (mACh) receptor subtypes, M1 and M2, on learning and memory, we examined the effects of three antagonists, atropine (non-selective), pirenzepine (M1 selective) and 11-[[2[(diethylamino)methyl]-1-piperidinyl]acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepine-6-one, AF-DX 116 (M2 selective), on stpe-through passive avoidance tasks in mice. Pre-training (5min before) administration of atropine (1-40 nmol) and pirenzepine (10 and 40 nmol) shortened the response latency in retention tests at 14 d after acquisition training. Pre-test (5min befor) and post-training (immediately after the acquisition) administration of atropine slightly but not significantly impaired retention scores. The administration of AF-DX 116 did not apparently affect the scores in any of tests. Thus, the M1 receptor subtype coupling systemes seem to be more important in the acquisition-consolidation process rather than in the retrieval process.