A study on in situ high density cultivation of anchorage dependent animal cells.

在普通方法中，依赖于锚定的动物细胞培养在固体培养基质上，如页岩或微珠的表面。这种处理可能会有有害的影响，例如细胞膜蛋白的蛋白质分解。为了避免这些影响，在本研究中使用了液-液界面系统的细胞培养。细胞生长在培养液和疏水性液体之间的界面上。与固体表面相比，界面上培养的动物细胞扩散较少，但系统具有原位条件。结果表明：(1)表面活性剂的数量和种类对细胞在界面上的黏附有影响；(2)细胞的形态受改变细胞在界面上黏附的表面活性剂的浓度控制；(3)细胞在界面上的铺展相对较小，从而使细胞在界面上锚定。并形成多细胞…(4)在乳状液表面进行培养，发现乳状液的直径分布要均匀，才能恢复细胞，防止彼此合并。(5)采用水包油和油包水两种体系的乳状液，培养效率均高于平板培养。Sato，M.，Hishinuma，T.，Terachi，K.，Hatori，T and Saito，M.：《绝缘液体和粘性液体在蒸馏水中的静电单分散》，《静电学‘95(第九届国际会议)》，P1.21，pp.65-66(York，1995)；Sato，M.，Hatori，T.和Saito M.：《液体-液体体系中静电分散形成液滴机理的实验研究》，Conf.Rec-IAS Annu.Meet(IEEE)，pp.1485-1492(Orlando，1995)；Sato，M.，Kato，S.和Saito，M.：“使用交流会聚电场产生O/W型均匀尺寸的液滴”，IEEE汇刊。Ind.App.，32,138-145(1996)；Sato，M.，Kudo，N.和Saito，M.，“使用振动喷射方法通过外加电场降低液体的表面张力”，会议参考-IAS Annu.Meet。(IEEE)，第1762-1767页(圣地亚哥，1996年10月)，更少

In an ordinary method, anchorage-dependent animal cells cultivated on a solid culture substrate, such as the surface of shale or micro-beads. This treatment may have detrimental effects such as the proteolysis of the cell membrane proteins. To avoid these effects, cell cultivation using a liquid-liquid interface system was used in the present study. The cells grow at the interface between a culture medium and a hydrophobic liquid. The animal cells cultivating at the interface spread less than the case of solid surface but the system Could have in situ conditions. In the present study, the following results were obtained :(1) Quantity and kind of the surface active agent affected to the adhesion of the cells at the interface.(2) Morphology of the cell was controlled by concentration of surface active agent which varied cell adhesion to the interface.(3) Cell spreading at the interfaces was relatively small, so that the cells at an interface anchored. with one another and formed multicel … More lular hemispherical aggregations shaped like a spheroid.(4) Cultivation on the emulsion surface was also tried, as a result, it was found that the diameter distribution of the emulsion should be uniform for the purpose of recovery of the cells and preventing the coalescence of each other^<**>.(5) Using emulsion with the systems of oil-in water and water-in-oil, cultivation efficiency was exceeded than the case of using flat plate in both cases.^<**>Sato, M., Hishinuma, T., Terachi, K., Hatori, T and Saito, M. : "Electrostatic mono dispersion of insulating and viscous liquid in distillled water, "Electrostatics'95 (9th International Conference), P1.21, pp.65-66 (York, 1995) ; Sato, M., Hatori, T.and Saito, M. : "Experimental investigation of droplet formation mechanisms by electrostatic dispersion in a liquid-liquid system, "Conf.Rec.-IAS Annu.Meet (IEEE), pp.1485-1492 (Orlando, 1995) ; Sato, M., Kato, S.and Saito, M. : "Production of O/W type uniformly sized droplets using AC convergent eletric field, "IEEE Trans. Ind.Appl., 32,138-145 (1996) ; Sato, M., Kudo, N., and Saito, M., "Surface tension reduction of liquid by applied electric field using vibrating jet method, "Conf.Rec.-IAS Annu.Meet. (IEEE), pp.1762-1767 (San Diego, October, 1996) Less