New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

肠道非特异性炎症性疾病-克罗恩病，溃疡性结肠炎和肠道白塞氏病-的原因尚不清楚。这些痛苦的补救措施是全胃肠外营养和类固醇和柳氮磺胺吡啶免疫抑制剂的管理。然而，众所周知，长期全胃肠外营养会导致局部免疫能力下降，并伴有肠道废用性萎缩。本研究的目的如下：（1）（a）以产生具有上级止血效果的可吸收EPA为目标的预备性开发。(b)通过使用卵磷脂建立乳化的准备技术，以及（c）通过混合大豆油和鱼油产生包含omega-3脂肪的乳液。(2)检查临时管理的有效性 关于我们 hod，通过在患病动物模型上使用新开发的脂肪营养素。(3)通过（a）向动物模型给予适当的鱼油混合物，（B）分析血清中的脂肪酸，（c）检查组织学结果和肠粘膜的生物功能状态，确认作为脂肪营养素的效果。结果：给予具有各种脂肪酸组成的TPN溶液。大豆含有6.9%的omega-3脂肪酸，鱼油含有32.1%的omega-3脂肪酸。各组中使用的脂肪乳剂及其相对比例如下：组I：仅大豆油乳剂;组II：75%大豆油和25%鱼油乳剂的混合物;组III：50%大豆油和50%鱼油乳剂的混合物;组IV：25%大豆油和75%鱼油乳剂的混合物;组V：仅鱼油乳剂;组VI：7天TPN期结束后，在乙醚麻醉下对大鼠进行剖腹手术。溃疡性结肠炎愈合程度通过结肠炎区域的HE染色和按照标准分级来评估，II组结肠炎的严重程度最轻（含有13.2%的ω-3脂肪酸），并且在组III，I，根据Dunnett多重比较，与VI组相比，采用Tukey多重比较法与V组比较。结果表明，给予ω-3必需脂肪酸具有抗炎作用。此外，结果表明，治疗效果不能预期从管理的脂肪乳剂只含有大豆油（组I）。进一步的研究是必要的，以确定适当的鱼油剂量之间的浓度。少

The causes of nonspecific inflammatory diseases of the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion of the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effects. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes omega-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration met … More hod, by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results ;TPN solutions with various fatty acid compositions were administered. Soybean contains 6.9% omega-3 fatty acid and fish oil containns 32.1% of omega-3 fatty acid. The fat emulsions and their relative proportions used in the respective group were as follows :Group I : Soybean oil emulsion only.Group II : mixture of 75% soybean oil and 25% fish oil emulsion.Group III : mixture of 50% soybean oil and 50% fish oil emulsion.Group IV : mixture of 25% soybean oil and 75% fish oil emulsion.Group V : fish oil emulsion only.Group VI : no fat emulsion administered.After completion of the 7-day TPN period the rats were laparotomized under ether anesthesia. The degree of healing of ulcerative colitis was assessed by HE staining of the colitis region and grading in accordance with the criteria.The severity of colitis was least in Group II (containing 13.2% of omega-3 fatty acid), and was relatively greater in Group III,I,IV and VI in that order.Group II displayd a significantly lower degree of severity as compared with group VI according to the Dunnett multiple comparison method and also as compared with group V according to the Tukey multiple comparison method. The result indicates that administration of omega-3 essential fatty acids has an anti-inflammatory effect. Moreover, the results indicated that healing effects could not be expected from administration of fat emulsions containing only soybean oil (Group I).Further studies are necessary in order to ascertain the concentrations between appropriate fish oil dosages. Less