Effect of mastification of derived brain factor

强化脑内基因表达的影响。咀嚼将其定义为饮食粉碎和下颌运动。探讨了咀嚼对大脑中枢的机制维持有怎样的影响。我分析了参与记忆学习的海马区。海马总RNA1ug采用RT-PCR法测定各RNA片段中BDNF-mRNA的含量。所有RNA1ug周期数均保持在20个左右，相对较好。它被巧妙地按周期数保存在20左右。由于EtBr检测的敏感性提高，我可以减少PCR的循环数。饲粮1次使BDNF/mRNA水平升高。大鼠海马中BDNF RNA水平随动量增加而升高。在给药大鼠的脑组织中，海马区、小脑、齿状回的基因表达差异无统计学意义。下丘脑、饱腹感中枢可见小表现。海马区BDNF mRNA表达量用生物成像分析仪测定，误差在30%左右。在该测定系统中，在再现性方面，切片之间的误差在30%左右，在紧急情况下误差最大。对咀嚼反应脑区基因表达的定量分析。

Effect of mastification of gene expression in brain.The mastification defined it as diet comminution and jaw movement.I referred to what kind of influence mastification had in mechanism maintenance of brain center. I analyzed in the hippocampus area which participated in anamnesis learning. Total RNA1ug from hippocampus did determination of the quantity of BDNF-mRNA in all RNA fraction by RT-PCR.It was kept by all RNA1ug cycle number around 20got from hippocampus smartly comparatively. It was kept by cycle number around 20 smartly.Because sensibility imprved as for detection by EtBr, I was able to reduce cycle number of PCR.A one of rat of diet increased a level of BDNF/mRNA.m RNA level of BDNF rose with gain of momentum in rat hippocampus.As for gene expression in brain of the rat which daiet was given to, the difference which was significance during hippocampus area and cerebellum, dentate gyrus wasn't recognized. Small manifestation was found in hypothalamus, satiety center. The quantity of mRNA manifestation of BDNF in hippocampus area came with determination with error of around 30% from 20 by using Bio Imaging Analyzer. In this determination system, error between section became clear in reproducibility in around 30% and an emergency by a maximum. Quantitative analysis of gene expression in area in the brain which replied to mastification response.