Effects of various drugs on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat

我们实验研究了促胰液素对雨果素诱导的急性胰腺炎大鼠胰腺外分泌功能的影响。在清醒大鼠胰腺外瘘手术准备后，检测了胆囊收缩素刺激的胰腺分泌。手术准备4天后，以10µg/kg/h的速率静脉注射雨蛙素4h，然后给予生理盐水(对照组)或促胰液素(5CU/kg/h，8h；促胰液素组)，诱导急性胰腺炎。分别于给药前一天和给药后一天进行胰腺外分泌功能检测。光镜下，对照组胰腺间质明显水肿，腺泡细胞内空泡化。这些发现在应用促胰液素后得到了部分改善。在对照组，基础状态和缩胆囊素刺激的胰液流量、碳酸氢盐排出量和蛋白质排出量在给药后显著减少。促胰液素重建了胰液流量和碳酸氢盐输出，并部分恢复了蛋白质输出。与未用促胰液素治疗的大鼠相比，在雨伞花素诱导的胰腺炎大鼠中，促胰液素可减轻胰腺组织病理学并恢复外分泌功能。

We experimentally investigated effect of secretin on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat. Cholecystokinin-stimulated pancreatic secretion was examined in conscious rats after surgical preparation with external pancreatic fistula. Four days after surgical preparation, we induced acute pancreatitis by intravenous administration of cerulein at a rate of 10 mug/kg/h for 4 hours, followed by administration of normal saline (control group) or secretin (5CU/kg/h, 8 hours ; secretin group). Pancreatic exocrine function test was undertaken one day before and after cerulein administration. Microscopically, the pancreas in control group showed prominent interstitial edema and intracellular vacuolization of the acinar cell. These findings were partially improved after secretin administration. In control group, basal and cholecystokinin-stimulated pancreatic juice flow, bicarbonate output, and protein output decreased significantly after cerulein administration. Secretin reestablished pancreatic juice flow and bicarbonate output, and partially restored protein output. In rats with cerulein-induced pancreatitis, secretin reduced pancreatic histopathology and restored exocrine dysfunction, when compared with non-secretin-treated rats.