CHEMOTAXIS OF HUMAN PERIODONTAL DERIVED CELLS

皮瓣反射完成后，软组织与根面接触。上皮和结缔组织的再附着在3周完成。牙龈固有层的自由活动细胞包括成纤维细胞、肥大细胞和少量单核和多形核白细胞。数量最多的细胞类型是成纤维细胞。人牙龈成纤维细胞（HGFs）产生胶原、蛋白聚糖、弹性蛋白等结缔组织物质，基本维持牙龈固有层的完整性。本研究评价了人牙龈成纤维细胞条件培养基（HGF- cm）对人牙周韧带成纤维细胞（HPLFs）、HGF与人牙槽骨细胞（HABCs）、人牙龈上皮细胞（HGEs）趋化性的影响。为了确定HGF-CM是否对成纤维细胞具有趋化和/或趋化动力学作用，对Zigmond-Hirsch型进行了棋盘分析，其中将不同浓度的HGF-CM添加到改良盲孔boyden型室的上（细胞）和下（测试）室，并测量成纤维细胞的迁移。这一分析表明，HGF- cm诱导HGF和HPLF的趋化迁移，因为在正梯度存在的情况下，细胞迁移增加，即肽浓度为175ng/ml时，下腔室比上腔室高。当HGF-CM浓度高于50%时，HGF-CM持续增加HABC和HGE的迁移，与阳性对照HPLF-CM（100%）结果相似。这些数据表明HGF-CM可能有助于牙周再生的发展。

At the completion of flap reflection, the soft tissues are placed in contact with the root surface. Epithelial and connective tissue reattachment is complete at 3 weeks. Freely mobile cells of the gingival lamina propria include fibroblast, mast cells, and a small number of mononuclear and polymorphonuclear leukocytes. The most numerous cell type is the fibroblast. Human gingival fibroblasts (HGFs) produce connective tissue substances including collagen, , proteoglycans, and elastin, and essentially maintain the integrity of gingival lamina propria. The present investigation was conducted evaluate the effect of human gingival fibroblasts conditioned medium (HGF-CM) to chemotaxis of human periodontal ligament fibroblasts (HPLFs), HGF and human alveolar bone cells (HABCs), and human gingival epithelial cells (HGEs).To determine whether HGF-CM was chemotactic, and/or chemokinetic for fibroblast, a checcurboard analysis of the Zigmond-Hirsch type was performed wherein different concentrations of HGF-CM were added to upper (cell) and lower (test) compartment of modified blind-well Boyden-type chamber, and migration of fibroblasts was measured. This analysis suggests that HGF-CM induced chemotactic migration of HGF and HPLF,because the cells migration increased in the presence of positive gradient, i.e.a higher of 175ng/ml concentration of the peptide in the lower compartment than in the upper compartment. HGF-CM consistently increased migration of HABC and HGE at concentration higher of 50% HGF-CM,similar result of HPLF-CM (100%) which was positive control. The data suggested that HGF-CM may contribute to the development of periodontal regeneration.