Molecular design of artificial glycoconjugate polymers and their applications to materials biotechnology

1.人工糖缀合物聚合物的分子设计已经开发了几种方便的合成具有重要生物学意义的复杂寡糖的路线，然后将这些寡糖转化为相应的糖聚合物。(1)以乳糖为原料合成了Gb 3-三糖（Galα1-4-Galβ1-4-Glcb），并将其转化为丙烯酰胺型单体物质，然后进行聚合。(2)采用简便的方法合成了甘露糖三糖，并将其引入到糖基共聚物中.糖共聚物的表征。(1)通过与凝集素的相互作用研究了糖识别中的簇效应。(2)通过p-A等温线和表面等离子体共振技术研究了碳水化合物之间的相互作用.材料生物技术的应用（1）携带唾液酸乳糖的糖聚合物对流感病毒的捕获（2）携带Gb 3-三糖的糖聚合物对大肠杆菌O-157毒素的中和（3）携带硫酸化半乳糖的糖聚合物对抗体的生产

1. Molecular design of Artificial Glycoconjugate PolymersSeveral convenient synthetic routes of biologically important, complex oligosaccharides have been developed and then these oligosaccharides have been converted to the corresponding glycopolymers.(1) Gb3-trisaccharide (Galα1-4-Galβ1-4-Glcb) was synthesized from lactose and converted to acrylamide-type monomeric substance, followed by polymerization.(2) Mannose trisaccharide was synthesized by a convenient route and introduced to glycopolymer.2. Characterization of the glycopolymers.(1) Cluster effect in glyco recognition was investigated by the interactions with lectins.(2) Carbohydrate-carbohydrate interaction was investigated by p-A isotherms abd surface plasmon resonance techniques.3. Applications to Materials Biotechnology(1) Trapping of influenza viruses by sialyl-lactose-carrying glycopolymer(2) Neutralization of E-coli O-157 toxins by Gb3-trisaccharide-carrying glycopolymer(3) Production of antibodies by sulfated-galactose-carrying glycopolymers