Detection of Biologically Active Substances by Chemical Reception

淡水腔肠海蛇在还原型谷胱甘肽的刺激下表现出摄食行为。行为反应被沐浴介质中的少量生物活性物质所改变。每种物质在不同的刺激条件下观察其修饰情况。首先，我们研究了培养条件，在这种条件下，生物活性物质的修饰可以重复性观察。我们发现，孵化猎物的孵化介质中的盐成分对于改变九头蛇的反应非常重要。由于水蛇行为测试对生物活性多肽非常敏感，因此有望用于研究生物标本中的这些物质，有时这些物质只有少量可用。我们在强迫游泳后疲劳大鼠的脑脊液中发现了转化生长因子-β活性。在另一项研究中，我们发现奎宁刺激口腔后，大鼠脑脊液中的安定结合抑制物(DBI)增加。与其他检测生物活性多肽的研究方法相比，我们可以利用这种水合反应系统更容易地检测到生物活性多肽中的这些变化并对其进行鉴定。这些例子表明，九头蛇反应在检测少量生物样本中的生物活性物质方面是有用的。目前，我们通过双筒观察行为来检查九头蛇反应，并确定反应。都是人的东西。这使得化验变得非常困难。然后我们开发了一个用于检测的计算机辅助系统。我们已经完成了必要的计算机程序的开发，以获取从CCD摄像机获得的数字图像，计算图像参数，并对这些图像参数进行分析。

A fresh water coelenterate Hydra shows the feeding behavior after stimulation by reduced glutathione. The behavioral response is modified by a small amount of biologically active substances in the bathing medium. The modification is observed at different stimulatory conditions by each substance. First, we have investigated the cultural conditions under which the modification by biologically active substances is observed reproducibly. We have found salt compositions of the hatching medium, in which prey is hatched, are important for the modification of the hydra response.Since the hydra behavioral test is so sensitive to biologically active peptides, it is expected to be useful for the investigation of these substances in the biological specimens, which sometimes are available only small amount. We found a TGF-beta activity in the rat cerebrospinal fluid (CSF) of the fatigued rat after forced swimming. In another study, we found the diazepam binding inhibitor (DBI) increased in the rat CSF after quinine stimulation to the oral cavity. We can detect these changes in the biologically active peptides and identify the peptide using this hydra response system easier than with other investigation methods for the detection of biologically active peptides. These examples show usefulness of the hydra response to examine biologically active substances in the small amount of biological samples.Currently, we examine the hydra response by observing the behavior by a binocular and determine the response. All are human things. This makes the assay to be very difficult to practice. Then we develop a computer-assisted system for the assay. We have completed to develop necessary computer programs to acquire digital images from a CCD camera, to calculate image parameters, and analyze these image parameters.