The localization of apoptosis and apoptosis-related genes (bcl-2,p53) expression

采用免疫染色和TUNEL法研究非黑色素瘤皮肤癌(鳞状细胞癌（SCC, n=4）、鲍文病（BD, n=5）、光化性角化病（AK, n=3）、基底细胞癌（BCC, n=5）中凋亡及凋亡相关基因（p53, bcl-2）的定位。将结果与PCNA免疫染色测定的增殖活性和形态分化程度进行比较。SCCs的角化异常细胞、BD和AK病变的分化部位以及BCC的部分肿瘤细胞中均检测到凋亡细胞。相反，PCNA在SCCs、bcc的许多肿瘤细胞和BDs的大部分肿瘤细胞中呈阳性。在AK病变中，基底细胞和基底上细胞PCNA阳性。结果表明，细胞凋亡过程与鳞状增生性肿瘤的分化有关。在BD和AK病变的增殖部分可见p53阳性细胞。在SCC中，p53阳性的肿瘤细胞较多，而在BCC中只有少数，尽管p53的表达程度存在差异。这些结果提示，在SCC中，BD和AK p53的表达与肿瘤细胞的增殖有关。在BCC中，p53也可能参与肿瘤细胞的发育。PCNA与p53表达之间没有一致的相关性。bcl-2在大多数SCC、BD、AK及邻近的正常表皮中均未表达，但在1例BD的基底细胞中检测到bcl-2的微弱表达。在5例BCC中，4例肿瘤细胞中检测到bcl-2的弥漫表达。这些结果提示bcl-2可能在BCC的发生发展中起重要作用。我没有发现bcl-2、PCNA的表达与细胞凋亡之间的关系。非黑色素瘤皮肤癌细胞凋亡的机制有待进一步研究。

The localization of apoptosis and apoptosis-related genes (p53, bcl-2) in non-melanoma skin cancers(squamous cell carcinoma(SCC, n=4), Bowen's disease(BD, n=5), actinic keratosis(AK, n=3), Basal cell carcinoma(BCC, n=5) was investigated using immunostaining and the TUNEL method. The resulting locations were compared with those of proliferative activities determined by PCNA immunostaining and the degree of morphological differentiation. Apoptotic cells were detected among the dyskeratotic cells of SCCs, the differentiated parts of BD and AK lesions and some tumor cells of BCC. On the contrary, PCNA was positive in many tumor cells of SCCs, BCCs and most tumor cells of BDs. In the lesions of AK, basal and suprabasal cells were positive with PCNA. There results indicate that apoptotic process is related to the differentiation of squamoproliferative tumors. P53-positive cells were observed in the proliferative portion of BD and AK lesions. In SCC, more tumor cells were p53-positive compared to only a few in BCC, although there are variations of the degree of p53 expression. These results suggest that in SCC, BD and AK p53 expression is related to the proliferation of tumor cells. In BCC p53 also might be involved in the development of tumor cells. There is no consistent correlation between PCNA and p53 expression. No bcl-2 expression was observed in most cases of SCC, BD, AK and adjacent normal epidermis, although weak bcl-2 expression was detected in the basal cells in one case of BD. In 4 of 5 cases of BCC the diffuse expression of bcl-2 was detected in the tumor cells. These results suggest that bcl-2 may play an important role of the development of BCC. I could not find any relationships between the expression of bcl-2, PCNA and apoptosis. Further studies are needed to elucidate the mechanisms of apoptosis in non-melanoma skin cancer.