Analysis of factors associating to parasitism and vaccine development of re-emerging Parasite, Echinococcus, using the alternative definitive host model

为了改进Echinococcus多用途、不同种类的类固醇和不同的剂量、站点、周期和频率的行政管理与Mongolian Gerbils相比较。最常见的蠕虫因后续组合而产生的副作用较少;类固醇：三丁基乙酸前二酮（PTBA），剂量：5毫克/头，站点：亚变化，周期：在感染结束试验前1周，频率：每一天。与吉尔吉斯的菌株比较表明了更多的蠕虫已经从我们的实验室中恢复了吉尔吉斯的菌株，比商业上可用的两条弯曲菌株。在hamster中， CN inbred strain吸收了比CAN strain更多的蠕虫。功能免疫力对寄生虫的建立不能被指定。环孢子素A或亚拉奇多离子级联不同阻断器的增加没有改善模型动物中的蠕虫恢复，而PTBA做了什么。不幸的是，蠕虫恢复的改进没有通过使用Mast Cell Deficient W/W D1 vieD 1 mice来实现。该模型可以成功地应用于E， Vogeli。在这个模型中，寄生虫蛋在35天后感染（DPI）处形成了特别的表格，寄生虫在50 DPI处被恢复，以分析对成人的保护免疫力。仓鼠中的多聚氰胺，再感染和口腔免疫研究已经被带走了。在挑战性感染后，蠕虫恢复在再感染组中明显减少，但不在口腔免疫组中。在再感染组中，增加了特定血清IgG， IgA和肠道IgA保密被观察到，在口头免疫组中，只有血清IgG增加。这些结果表明，对成人E.产生的抵抗力，多聚氰胺可能与寄生虫抗原的系统和局部识别有关，特别是在IgA反应中。

To improve the alternative definitive host model for Echinococcus multilocularis, different kinds of steroids, and different doses, sites, periods and frequencies of administration were compared using Mongolian gerbils. The most worms with less side effect were recovered by the following combination; steroid : prednisolone tertiary-butylacetate (PTBA), dose: 5mg/head, site: subcutaneous, period: from 1 week before infection to the end of the experiment, frequency: every other day. Strain comparison of gerbils indicated more worms were recovered from the gerbils raised in our laboratory than commercially available two inbred strains. In hamster, CN inbred strain harbored more worms than CAN strain.Functional immunity against the establishment of the parasites could not be specified. Adiministration of cyclosporin A or different blockers of arachidonic cascade did not improve the worm recovery from the model animals as that of PTBA did. Nor, improvement of worm recovery was not achieved by using mast cell deficient W/WィイD1vィエD1 mice.The model could be successufully applied to E, vogeli. In this model, the parasite eggs was excreted form gerbils at 35 days post infection (DPI) and mature parasites were recovered at 50 DPI.To analyze protective immunity against adult E. multilocularis in hamsters, re-infection and oral immunization studies were carried out. Worm recovery after challenge infection was significantly reduced in the re-infection groups but not in the oral immunization groups. In the reinfection groups, increase of specific serum IgG, IgA and intestinal IgA secretion was observed, however, in the oral immunization groups, only serum IgG increased. These results indicated that acquired resistance against adult E.multilocularis may be associated with systematic and local recognition of the parasite antigen, especially in IgA reaction.