Bioremediation of dioxins with dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi

利用二恶英降解真菌及其原生质体融合形成的融合体对土壤和焚烧灰中的二恶英进行生物修复降解。同时，还形成了对真菌融合产生的二恶英具有较高降解能力的融合体。土壤中的每一种二恶英（2,7 - dcdd、2,4,8 - tcdf和2,3,7,8 - tcdd）都可以通过二恶英降解真菌进行生物修复。各二恶英降解率分别为70%、65%和60%，持续30 d。利用二恶英降解真菌对水田土壤进行30 d生物修复，可降解二恶英约90%。此外，生物修复还消除了土壤中二恶英的毒性。此外，在生物修复过程中，表面活性剂的加入提高了二恶英的降解程度。用可降解二恶英的真菌对烟灰进行60天的生物修复，降解了约50%的二恶英。经反复生物修复后，灰渣中二恶英含量明显增加。水稻田土壤中的二恶英可通过真菌原质体融合形成的融合体进行生物修复降解。真菌对二恶英的降解程度略高于生物修复法。此外，在重复传代培养中，融合体降解二恶英的能力下降。

Biodegradation of dioxins in soils and incinerated ashes by bioremediation was conducted by use of dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi. And, formation of fusants having higher ability for degradation of dioxins formed by the fusion of the fungi were also conducted.1. Each dioxin (2, 7-DCDD, 2, 4, 8-TCDF and 2, 3, 7, 8-TCDD) in soils could be degraded by bioremediation with dioxins degradable fungi. Each dioxin was degraded about 70%, 65% and 60%, respectively, for 30 days.2. About 90% of dioxins in paddy filed soils was degraded by bioremediation with dioxins degradable fungi for 30 days. The toxicity of the dioxins in the solis was alos removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased by addition of a surfactant during the bioremediation.3. About 50% of dioxins in nicinerated ashes was degraded by bioremediation with dioxins degradable fungi for 60 days. It was found that the degree of the dioxins in the ashes was considerably increased by repetition of the bioremediation.4. Dioxins in paddy filed soils could be degraded by bioremediation with fusants formed by prptoplast fusion of the fungi. The degree of degradation of the dioxins was a little higher than that by the bioremediation with the fungi. In addition, the ability of fusants for degradation of dioxins decreased in the repeated subcultures.