The analysis of expressed genes in fish injected with immunostilumant

从注射肽聚糖的牙鲆肾脏中获得表达序列标签（ESTs）。将提供来自肾cDNA的198个克隆的EST的单程测序结果。将cDNA克隆的序列与GenBank数据库中的序列进行比较。808个克隆（44.4%）似乎是完全未知的，并且可能代表新描述的基因，而110个克隆（55.6%）基于与数据库中的序列的匹配而被鉴定。结果包括α珠蛋白、几种核糖体蛋白、α胶原、热休克蛋白90和细胞色素氧化酶亚基等基因。从肾脏中鉴定的免疫相关cDNA为免疫球蛋白重链和轻链、β2-微球蛋白、胸腺素β和溶菌酶、MHC 11α类。

Expressed sequence tags (ESTs) were obtained from the kidney of Japanese flounder Paralichthys olivaceus injected with peptidoglycan. The results of single-pass sequencing of ESTs from 198 clones from kidney cDNA will be presented. Sequences of the cDNA clones were compared with sequences in the GenBank database. Eighty and eight clones (44.4 %) appeared to be completely unknown and are likely to represent newly described genes, whereas 110 clones (55.6%) were identified based on matches to sequences in the database. The results contain the genes such as alpha globin, several ribosomal proteins, alpha collagen, heat shock protein 90 and cytochrome oxidase subunit. Immune related cDNAs identified from the kidney were immunoglobulin heavy and light chain, β2-microglobulin, thymosin β and lysozyme, MHC class 11α.