Myocardial ischemia imaging using monoclonal antibodies

利用心肌膜联蛋白单克隆抗体检测心肌缺血区域小林英树、单桃光、KUSAKABE清子、kanekonoboru东京女子医科大学放射学系本研究的目的是阐明使用^<111>In标记的抗膜联蛋白V抗体成像检测心肌缺血区域的可能性。方法Wistar大鼠进行短暂性急性缺血（左冠状动脉闭塞10 min后再灌注），缺血组（N=31）和对照组（N=29）。模型制备后第2、7、14、21、50天分别注射7.4 MBq ^<111>In标记的抗膜联蛋白V抗体。结果缺血组第2、7、14天的心肺比（3.32±0.21、3.03±0.2 1、3.19±0.64）明显高于对照组（1.41±0.51、1.14±0.36、1.40±0.52）（p<0.01）。缺血组大鼠在造模50 d后，第2、7、14天的心肺比（2.03±0.25）均较缺血组降低。大x线自显像显示缺血组左冠状动脉前降区明显^<111 bb0 In标记抗膜联蛋白V抗体积累。显微镜检查显示缺血组无心肌梗死区。结论^<111>标记抗膜联蛋白V抗体显像能清晰地检测出该模型10 min后的心肌缺血区域。抗膜联蛋白V抗体显像可提供心肌缺血记忆的独特信息。

DETECTION OF MYCARDIAL ISCHHEMIC AREA USING MONOCLONAL ANTIBODIES FOR MYOCARDIAL ANNEXIN VHideki KOBAYASHI, Mitsuru MOMOSE, Kiyoko KUSAKABE, Noboru KanekoDepartment of Radiology, Tokyo Woman's Medical UniversityThe purpose of this study was to clarify the possibility of detection of myocardial ischemic area using ^<111>In labeled anti-annexin V antibody imaging. Methods Wistar rats were subjected to transient acute ischemia (10 min of left coronary occlusion and following reperfusion, ischemia group, N=31) and controls (control group, N=29). Anti-annexin V antibody labeled with 7.4 MBq of ^<111>In was injected 2, 7, 14, 21 and 50 days after the model preparation. Results Heart/lung ratio in the ischemia group on days 2, 7 and 14 (3.32±0.21, 3.03±0.2 1, 3.19±0.64) was significantly higher (p<0.01) than that in the control group (1.41±0.51, 1.14±0.36, 1.40±0.52). Fifty days after the model preparation in the ischemia group, the heart/lung ratio (2.03±0.25) was decreased compared with that in the ischemia group on days 2, 7 and 14. Macroautoradiography showed significant ^<111>In labeled anti-annexin V antibody accumulation in the left anterior descending coronary territory in the ischemia group. Microscopic examination showed no myocardial infarct area in the ischemia group. Conclusion ^<111>In labeled anti-annexin V antibody imaging clearly detected the myocardial ischemic area following 10 min of transient acute ischemia in this experimental model. Anti-annexin V antibody imaging can provide unique information regarding the ischemic memory of myocardium.