Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin V

使用心肌膜联蛋白 V 单克隆抗体检测阿霉素诱导的心肌细胞损伤

• 批准号: 13670969
• 负责人: KOBAYASHI Hideki
• 金额: $ 1.22万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670969/
• 关键词: anti-Annexin V antibody; imaging; 抗体イメージング; アドリアマイシン心筋症; 早期発見; アドリアマイシン心筋症モデル; イメージング

Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin VAnnex in V, a calcium-dependent phospholipid-binding protein that exists in the soluble fraction of myocardium, was translocated from the soluble fraction of myocardium to the myocardial cell membrane when the myocardium was damaged in the patients with congestive heart failure. The purpose of this sturdy was to clarify the diagnostic value of In-111 labeled Monoclonal Antibodies for Myocardial Annexin V for the detection of damaged myocardium. Methods. Forty Wister rats were injected 0.2mg of doxirubicin weekly for making doxirubicin induced cardiomyopathy model. In-111 labeled anti-Annexin V antibody was injected 4, 6, 8 and 9 weeks after the doxirubicin Injection. Results. Heart/Lung ratio in doxirubicin group on weeks 4, 6, 8 and 9 weeks (2.56±0.48, 2.30±0.49, 2.20±0.23, 0.85±0.41) were significantly higher than that in control group (1.30±0.21, 1.32±0.38, 1.39±0.23). Biodistribution analysis also showed that In-111 labeled anti-Annexin V antibody accumulation was higher in doxirubicin group than that in control group. Conclusion. In-111 labeled anti-Annexin V antibody scintigraphy positively imaged damaged myocardium in doxirubicin induced rat cardiomyopathy model.

心肌Annexin VAnnex in V单克隆抗体检测多昔比星诱导心肌细胞损伤，该蛋白是一种存在于心肌可溶部分的钙依赖性磷脂结合蛋白，在充血性心力衰竭患者心肌受损时从心肌可溶部分转移到心肌细胞膜上。本实验的目的是阐明In-111标记的心肌膜联蛋白V单克隆抗体在检测受损心肌中的诊断价值。方法。40只Wister大鼠每周注射多柔比星0.2mg，制作多柔比星致心肌病模型。在多柔比星注射后4、6、8、9周注射In-111标记抗膜联蛋白V抗体。结果。多柔比星组在第4、6、8、9周的心肺比（2.56±0.48、2.30±0.49、2.20±0.23、0.85±0.41）显著高于对照组（1.30±0.21、1.32±0.38、1.39±0.23）。生物分布分析还显示，多柔比星组in -111标记的抗膜联蛋白V抗体积累量高于对照组。结论。in -111标记抗膜联蛋白V抗体显像对多柔比星诱导的大鼠心肌病模型损伤心肌的显像呈阳性。