Endogenous gibberellins in developing grape berry and induction of seedlessness berry using them

1.从坎贝尔早熟葡萄、特拉华葡萄和科苏葡萄未成熟种子中提取内源赤霉素(GAs)。经高效液相色谱分析，3个品种表现出GAS活性的组分分别为14-15(I)、18-19(II)、23-25(III)和26-27(IV)。然而，总天然气活动早些时候在坎贝尔较高，其次是特拉华州和科斯尤。经GC-MS鉴定为GA_4、3-epi-GA_4、GA_7和GA_(34)。在花序盛开前2周和盛花后10天分别喷施GA_3、GA_4或GA_7。这些气体诱导了坎贝尔早期葡萄、特拉华葡萄和科苏葡萄的单性结实。但不同品种处理后的气调效果不同。浆果的比例更高。坎贝尔早、特拉华和科斯尤分别为GA_7(96%)和GT；GA_3(71%)和GT；GA_4(66%)；GA_7(99%)和GT；GA_4(97%)和GT；GA_3(94%)和A_3(28%)&GT；GA_7(16%)和GT；GA_4(14%)。此外，在坎贝尔和特拉华州早期使用GA_3、GA_4或GA_7对花粉萌发有强烈的抑制作用，但在Kosyuu没有观察到这种趋势。从未成熟的Campbell Elear和Kosyu种子中提取的气体用于两个品种开花期的去势小花。它们诱导单性结实，但不同品种的座果率不同。

1. Endogenous giberellins (GAs) were extracted from immature seeds of Campbell Early, Delaware and Kosyu grapes. After ODS-HPLC, the fractions that showed GAs activity in three cultivars were No.14-15 (I), No.18-19 (II) No.23-25 (III) and No.26-27 (IV). However, total GAs activity was higher in Campbell Early followed by Delaware and Kosyu. GAs present in IV of Campbell Early were identified as GA_4, 3-epi-GA_4, GA_7 and GA_<34>, by GC-MS.2. GA_3, GA_4 or GA_7 were appllied to flower cluster 2 weeks before and 10 days after full bloom. These GAs induced parthenocarpy in Campbell Early, Delaware and Kosyu grapes. However, the effect of the treated GAs was different among cultivars. Higher percentages of berry set in. Campbell Early, Delaware and Kosyu were observed in order GA_7(96%)>GA_3(71%)>GA_4(66%), GA_7(99%)>GA_4(97%)>GA_3(94%) and A_3(28%)>GA_7(16%)>GA_4(14%), respectively. And also, the aplication GA_3, GA_4 or GA_7 to Campbell Early and Delaware strongly inhibited the pollen germination, but such a trend was not observed in Kosyu.3. GAs extracted from immature seeds of Campbell Early and Kosyu were applied to the emasculated florets of both cultivars at anthesis. They induced parthenocarpy, but the percentage of berry set varied between cultivars.