Establishment of support network system for the technology of gene alternated mice production and embryonic manipulation by closer cooperation with academic and industrial fields

该项目的目的是通过与学术界和工业界的密切合作，建立基因交替小鼠生产和胚胎操纵技术的支持网络系统。首先，我们尝试开发转基因小鼠的胚胎操作和核移植技术。本项目建立了ALS模型大鼠、OGP转基因小鼠和人类朊病毒基因敲入小鼠的朊病毒检测系统。此外，我们还对兔胚胎冷冻保存技术和大鼠ICASI技术进行了基础研究。另一方面，我们研究所的大多数小鼠都感染了MHV。因此，我们通过与实验动物技术公司更密切的合作来应对。在这次事件中，我们与我们的学术机构和行业机构验证了合作网络系统。通过该项目，为建立实验动物技术支持网络系统与学术界和产业界的紧密合作提供了基础数据。

The aim of this project is to establish the support network system for the technology of gene-alternated mice production and embryonic manipulation by closer cooperation with academic and industrial fields.First we tried to develop the technology for embryonic manipulation of transgenic mice production and nuclear transfer. In this project we developed the ALS model rat, OGP transgenic mice and the prion assay system by human prion gene knock-in mice. In addition, we did basic research of technologies for rabbit embryo cryopreservation and ICASI technology for rat.On the other hand, we suffered from MHV infection to most of mice in our institute. Therefore we countered by the closer cooperation with the companies for laboratory animal technology. In this incident, we verified the cooperation network system with our academic institution and industrial institutions.By this project, we obtained the basic data for establishing the support network system for the technology of laboratory animals by closer cooperation with academic and industrial fields.