Influence of electrical and electromagnetic stimulation on peripheral nerve regeneration

采用小鼠坐骨神经损伤模型，从形态学上观察电、电磁刺激对神经再生的影响。切断后2天，对损伤的坐骨神经施加电和电磁刺激。在再生的神经纤维中，只有生长锥被选择性破坏，但再生纤维的轴突轴似乎是正常的。为观察电刺激对再生神经纤维成熟的影响，每天对再生坐骨神经进行电刺激和电磁刺激，并于刺激开始后第7、14、21和28天，在坐骨神经损伤端远端5mm处计数再生神经纤维的直径和数量。在第7天和第14天，刺激组的有髓再生纤维的数量和直径均多于对照组（未刺激）。结果表明，电、电磁造孔促进了再生神经纤维的延伸和成熟。但在第28天，刺激组的再生神经纤维数量明显少于对照组。

Influence of the electrical and electromagnetic stimulation on nerve regeneration was morphologically examined in the injured mouse sciatic nerve. Two days after the transection, the electrical and electromagnetic stimulation was applied to the injured sciatic nerve. In the regenerating nerve fibers, only the growth cones were selectively damaged, but the axonal shaft of the regenerating fibers appeared to be normal. To examine the influence of the stimulation on maturation of regenerating nerve fibers, electrical and electromagnetic stimulation was applied every day to the regenerating sciatic nerves, and diameter and the number of the remyelinated regenerating nerve fibers were counted at 5mm distal to the crush end of the sciatic nerve on 7th , 14th , 21th and 28th days after the begining of the stimulation. On the 7th and 14th days, the number and diameter of the remyelinated regenerating fibers in the stimulated groups were more than in the control group (without stimulation). It is suggested that the rate of extension and maturation of the regenerating nerve fibers are promoted by the electrical and electromagnetic stomulation. But, on 28th day, the number of the regenerating nerve fibers in the stimulated groups was much less than that in the control group.